DNA integrity-protecting and survival-promoting activity of serotonergic system in sturgeon juveniles and sazans
DNA integrity-protecting and survival-promoting activity of serotonergic system in sturgeon juveniles and sazans
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鲟鱼幼鱼和鲇鱼血清素能系统的 DNA 完整性保护和生存促进活性
DOI：
10.1007/s10695-017-0360-5
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发表时间：
2017
影响因子：
2.9
通讯作者：
S. K. Movsum
中科院分区：
文献类型：
--
作者：
A. Mekhtiev;Turan N. Allahverdiyeva;S. K. Movsum
该研究涉及评价在不利条件下的抗突变保护和存活机制中的α-胡萝卜素能系统的参与。结果表明，在长期暴露于石油和工业污染下，同时观察到鲟鱼幼鱼（Acipenser gueldenstaedtii persicus）肝脏中红细胞突变水平的急剧增加和抗整合蛋白（SMAP; Mekhtiev 2000）的下调。通过微核分析评价突变水平，通过使用抗SMAP免疫球蛋白的间接ELISA试验评价SMAP水平。肌肉注射SMAP可显著降低暴露于多环芳烃和重金属污染沉积物中的鲟鱼幼鱼红细胞微核率。通过低剂量的杀虫剂阿克他拉（100 mg/l）预处理萨赞导致SMAP的上调和保持在高浓度阿克他拉（400 mg/l）下的实验组中的所有动物的存活，而对照组中的所有动物在这些条件下死亡。I.M.与对照相比，在将萨赞放入含有高水平阿克他拉（400 mg/l）的水中之前施用SMAP导致它们的总存活。参与的抗突变保护和促进生存的破坏条件下的机制，β-肾上腺素能系统的结论。
The study concerns evaluation of engagement of serotonergic system in the mechanisms of antimutagenic protection and survival under adverse conditions. It is shown that under long-term exposure to oil and industrial pollution, simultaneous sharp increase of mutations level in the erythrocytes and downregulation of serotonin-modulating anticonsolidation protein (SMAP; Mekhtiev 2000) in the livers of the sturgeon juveniles (Acipenser gueldenstaedtii persicus) is observed. Mutation level was evaluated by the micronucleus analysis and SMAP level—by the indirect ELISA-test utilizing anti-SMAP immunoglobulins. Intramuscular administration of SMAP leads to significant decrease of micronucleus amount in the erythrocytes of the sturgeon juveniles exposed to sediments polluted with PAH and heavy metals. Pre-conditioning of sazans by low dose of insecticide actara (100 mg/l) leads to upregulation of SMAP and to survival of all the animals in the experimental group, kept under high concentration of actara (400 mg/l), while all animals in the control group succumbed under these conditions. I.m. administration of SMAP prior to putting the sazans into the water containing high levels of actara (400 mg/l), in contrast to controls, leads to their total survival. Participation of serotonergic system in the mechanisms of antimutagenic protection and survival promotion under damaging conditions is concluded.