Genome-Wide Identification, Classification and Expression Analysis of the MYB Transcription Factor Family in Petunia.
Genome-Wide Identification, Classification and Expression Analysis of the MYB Transcription Factor Family in Petunia.
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DOI：
10.3390/ijms22094838
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发表时间：
2021-05-03
影响因子：
5.6
通讯作者：
Pan J
中科院分区：
文献类型：
--
作者：
Chen G;He W;Guo X;Pan J
MYB转录因子的进化和功能一直是研究的热点。为了揭示矮牵牛花色多样性的形成机制，对矮牵牛MYB基因家族进行了鉴定和分析。在本研究中，共鉴定出155个MYB基因，其中1 R-MYB基因40个，R2 R3-MYB基因106个，R1 R2 R3-MYB基因7个，4 R-MYB基因2个。大多数R2 R3基因含有三个外显子和两个内含子，而PaMYB内含子的数量从0到12不等。R2 R3-MYB基因可分为28个亚群。基因结构和蛋白质基序分析显示，同一亚群内的成员呈现相似的外显子/内含子和基序组织，进一步支持系统发育分析的结果。亚组10、11和21基因主要在花瓣中表达，在营养组织中不表达。第9、19、25和27亚组基因在所有组织中均有表达，但各亚组基因的表达模式不同。启动子分析表明，5个R2 R3-MYB基因和2个MYB相关基因含有MBSI顺式元件，参与类黄酮生物合成调控。PaMYB 100/DPL对色素沉着有正调节作用。PaMYB 82、PaMYB 68和Pa 1 RMYB 36虽然含有MBSI顺式元件，但其在类黄酮生物合成中的作用尚未被揭示。与现有知识一致，亚组11中的PaMYBs与亚组6中的AtMYBs具有相似的功能，其中基因在花青素苷生物合成中起重要作用。此外，PaMYB 1和PaMYB 40属于P9（S7），可能参与矮牵牛营养器官类黄酮合成的调控。本研究为深入了解矮牵牛MYB基因家族的功能和进化奠定了基础。
A lot of researches have been focused on the evolution and function of MYB transcription factors (TFs). For revealing the formation of petunia flower color diversity, MYB gene family in petunia was identified and analyzed. In this study, a total of 155 MYB genes, including 40 1R-MYBs, 106 R2R3-MYBs, 7 R1R2R3-MYBs and 2 4R-MYBs, have been identified in the Petunia axillaris genome. Most R2R3 genes contain three exons and two introns, whereas the number of PaMYB introns varies from 0 to 12. The R2R3-MYB members could be divided into 28 subgroups. Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization, further supporting the results of phylogenetic analysis. Genes in subgroup 10, 11 and 21 were mainly expressed in petal, not in vegetative tissues. Genes in subgroup 9, 19, 25 and 27 expressed in all tissues, but the expression patterns of each gene were different. According to the promoter analysis, five R2R3-MYB and two MYB-related genes contained MBSI cis-element, which was involved in flavonoid biosynthetic regulation. PaMYB100/DPL has been reported to positively regulate to pigmentation. However, although PaMYB82, PaMYB68 and Pa1RMYB36 contained MBSI cis-element, their function in flavonoid biosynthesis has not been revealed. Consistent with existing knowledge, PaMYBs in subgroup 11 had similar function to AtMYBs in subgroup 6, genes in which played an important role in anthocyanin biosynthesis. In addition, PaMYB1 and PaMYB40 belonged to P9 (S7) and were potentially involved in regulation of flavonoid synthesis in petunia vegetative organs. This work provides a comprehensive understanding of the MYB gene family in petunia and lays a significant foundation for future studies on the function and evolution of MYB genes in petunia.