Identification of an amino terminal domain required for the transforming activity of the Rous sarcoma virus src protein.
Identification of an amino terminal domain required for the transforming activity of the Rous sarcoma virus src protein.
复制标题
劳斯肉瘤病毒 src 蛋白转化活性所需的氨基末端结构域的鉴定。
DOI：
10.1016/0042-6822(87)90011-0
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Parsons,JT
中科院分区：
文献类型：
--
作者：
Raymond,VW;Parsons,JT
Transformation of chicken cells by Rous sarcoma virus (RSV) requires the functional expression of the viralsrcprotein, a tyrosine protein kinase, pp60src. Variants of RSV containing deletions within the amino terminal one-third of thesrcprotein have been identified that exhibit either temperature-sensitive or transformation-defective phenotype when used to infect chicken embryo cells. To define the regions within the amino terminal portion of pp60srcthat influence morphological transformation, a series of overlapping deletion mutations in the src gene of Prague RSV (Pr A RSV) were constructed and their biological and biochemical properties were analyzed. Deletions within thesrcgene which remove amino acid residues 38 to 142 had minimal effects on the ability of the mutant viruses to induce cellular transformation. However, deletions, which impinged upon the region of the src gene encoding residues 142 to 169, inhibited cellular transformation. A variant containing a deletion of amino acid residues 169 to 225, was temperature sensitive for transformation. Structurally altered src proteins recovered from cells infected with transformation-defective variants exhibited a somewhat reduced tyrosine protein kinase activities when assayed in the immune complex kinase assay. Analysis of thein vivophosphorylation of a pp60srcsubstrate, the 36-kDa protein, revealed virtually wild-type levels of phosphorylation in cells infected with the transformation-defective mutants. These studies suggest that the region of the Pr A RSV src protein delineated by amino acid residues 142 to 169 is essential for initiation and maintenance of morphological transformation of chicken cells in culture.
