CHARACTERIZATION OF THE ESCHERICHIA-COLI GENE FOR 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (PLSC)
CHARACTERIZATION OF THE ESCHERICHIA-COLI GENE FOR 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (PLSC)
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DOI：
10.1007/bf00280009
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发表时间：
1992-03-01
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影响因子：
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通讯作者：
COLEMAN, J
中科院分区：
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作者：
COLEMAN, J
一株缺乏1-酰基-sn-甘油-3-磷酸酰基转移酶活性的大肠杆菌菌株已经被分离出来，并且该基因(PlsC)已被证明定位在染色体上最小65附近。我精确地定位了plsC在染色体上的位置，并测定了它的DNA序列。PLSC位于PARC和苏菲之间，与苏菲相隔74个BP。PlsC的上游是由233个碱基隔开的ParC，它包含一个活性启动子。ParC、plsC和Sufi都是在染色体上以逆时针方向转录的，可能是在有多个启动子的操纵子中。部分纯化的蛋白质的氨基末端序列与DNA序列相结合，表明1-酰基-sn-甘油-3-磷酸酰基转移酶是一种27.5 kDa的高度碱性蛋白质。PlsC基因产物1-酰基-sn-甘油-3-磷酸酰基转移酶定位于细胞膜。纯化蛋白的氨基末端序列显示，第一个氨基酸是封闭的蛋氨酸残基，最有可能是甲酰蛋氨酸。1-酰基-3-甘油-3-磷酸酰基转移酶的氨基酸序列与另外两种以酰基载体蛋白为酰基供体的大肠杆菌酰基转移酶有较短的同源性，分别是sn-甘油-3-磷酸酰基转移酶和UDP-N-乙酰氨基葡萄糖酰基转移酶(参与脂质A的生物合成)。
An Escherichia coli strain deficient in 1-acyl-sn-glycerol-3-phosphate acyltransferase activity has previously been isolated, and the gene (plsC) has been shown to map near min 65 on the chromosome. I precisely mapped the location of plsC on the chromosome, and determined its DNA sequence. plsC is located between parC and sufI, and is separated from sufI by 74 bp. Upstream of plsC is parC, separated by 233 bp, which includes an active promoter. parC, plsC, and sufI are all transcribed in the counterclockwise direction on the chromosome, possibly in an operon with multiple promoters. The amino-terminal sequence of the partially purified protein, combined with the DNA sequence, reveal 1-acyl-sn-glycerol-3-phosphate acyltransferase to be a 27.5 kDa highly basic protein. The plsC gene product, 1-acyl-sn-glycerol-3-phosphate acyltransferase, is localized to the cytoplasmic membrane of the cell. The amino-terminal sequence of the purified protein reveals the first amino acid to be a blocked methionine residue, most probably a formyl-methionine. The amino acid sequence of 1-acyl-sn-glycerol-3-phosphate acyltransferase has a short region of homology to two other E. coli acyltransferases that utilize acyl-acyl carrier protein as the acyl donor, sn-glycerol-3-phosphate acyltransferase and UDP-N-acetyl-glucosamine acyltransferase (involved in lipid A biosynthesis).