Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidian Ciona eggs.
Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidian Ciona eggs.
复制标题
DOI：
10.1091/mbc.e20-01-0083
复制
发表时间：
2021-02-01
影响因子：
3.3
通讯作者：
Tani T
中科院分区：
文献类型：
--
作者：
Ishii H;Tani T
合子细胞中细胞质的空间重组对于许多动物物种建立体型图至关重要。在海鞘受精卵中，母体决定因子（mRNAs）首先在受精后几分钟被运送到植物极，然后在第一次细胞分裂之前，在细胞质重组的后期阶段被运送到受精卵的未来后侧。在这里，通过使用一种新的荧光偏振显微镜，报告的位置和荧光标记的蛋白质在活细胞中的方向，我们映射的局部对齐和时间依赖性变化的皮质肌动蛋白网络在玻璃海鞘卵。最初的细胞质重组开始与收缩植物半球约20秒后受精诱导的[Ca 2 +]增加。植物收缩的时间是一致的高度对齐的肌动蛋白丝在植物半球的细胞皮层，垂直于动物-植物轴的出现。我们建议，细胞质重组启动的植物半球，这反过来又产生了定向运动的细胞质内的整个鸡蛋横向排列的皮质肌动球蛋白的局部收缩。
Spatial reorganization of cytoplasm in zygotic cells is critically important for establishing the body plans of many animal species. In ascidian zygotes, maternal determinants (mRNAs) are first transported to the vegetal pole a few minutes after fertilization and then to the future posterior side of the zygotes in a later phase of cytoplasmic reorganization, before the first cell division. Here, by using a novel fluorescence polarization microscope that reports the position and the orientation of fluorescently labeled proteins in living cells, we mapped the local alignments and the time-dependent changes of cortical actin networks in Ciona eggs. The initial cytoplasmic reorganization started with the contraction of vegetal hemisphere approximately 20 s after the fertilization-induced [Ca2+] increase. Timing of the vegetal contraction was consistent with the emergence of highly aligned actin filaments at the cell cortex of the vegetal hemisphere, which ran perpendicular to the animal–vegetal axis. We propose that the cytoplasmic reorganization is initiated by the local contraction of laterally aligned cortical actomyosin in the vegetal hemisphere, which in turn generates the directional movement of cytoplasm within the whole egg.
影响因子：
7.8
作者：
Speksnijder, J E;Sardet, C;Jaffe, L F
通讯作者：
Jaffe, L F