Generation of gene knockout mice by ES cell microinjection.
Generation of gene knockout mice by ES cell microinjection.
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通过ES细胞显微注射产生基因敲除小鼠。
DOI：
10.1002/0471143030.cb1914s44
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发表时间：
2009
影响因子：
--
通讯作者：
Kulkarni,AshokB
中科院分区：
文献类型：
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作者：
Longenecker,Glenn;Kulkarni,AshokB
本单元描述了用于生产嵌合体小鼠的方案，这些嵌合体小鼠随后用于基因敲除小鼠的生成。这些方案包括收集囊胚，注射ES细胞，以及注射的囊胚的子宫移植。还包括对胚泡供者小鼠的超数排卵、假孕受者的生成、用于胚胎和细胞操作的玻璃管的制造以及生殖系小鼠的生成的支持方案。文中提到了一些实用的提示和解决方案，以帮助解决可能出现的问题。普罗托克。细胞生物。44：19.14.1。-19.14.36。©2009 John Wiley&Sons，Inc.
This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols for the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipets for embryo and cell manipulations, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur.Curr. Protoc. Cell Biol. 44:19.14.1.‐19.14.36. © 2009 by John Wiley & Sons, Inc.