Quantitative analyses of neuroepithelial cell shapes during bending of the mouse neural plate.
Quantitative analyses of neuroepithelial cell shapes during bending of the mouse neural plate.
复制标题
小鼠神经板弯曲过程中神经上皮细胞形状的定量分析。
DOI：
10.1002/cne.903420113
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Quan,J
中科院分区：
文献类型：
--
作者：
Smith,JL;Schoenwolf,GC;Quan,J
尽管有大量关于鸡胚胎神经形成的细胞行为的信息，但小鼠胚胎中的类似行为尚未得到很好的表征。这项研究检查了小鼠神经板弯曲过程中发生的细胞行为，特别是神经上皮细胞形状的定性和定量变化。我们目前的结果表明，在小鼠胚胎中（1）中位铰接点（MHP），神经板的一个局部区域，锚定到下面的脊索前板中胚层或脊索/脊索板，并形成中线纵向沟槽，其余神经板（即，不参与 MHP 形成的神经板部分）的折叠围绕该沟槽发生，在神经折叠抬高阶段发育； (2) MHP 富含楔形神经上皮细胞，但与相邻的成对神经上皮侧面区域相比，纺锤形、倒楔形和球状神经上皮细胞明显较少 (L)； (3)每个L都富含纺锤形、倒楔形和球状神经上皮细胞，但楔形神经上皮细胞明显少于MHP。因此，神经上皮细胞的楔入发生在小鼠神经板弯曲期间，并且在神经褶皱抬高期间定位于MHP。同样，之前对小鸡的研究表明，神经上皮细胞在神经板弯曲期间变成楔形，并且这种细胞楔形在神经褶皱抬高期间定位于 MHP。此类研究还揭示了雏鸡 MHP 形成和 MHP 内神经上皮细胞局部楔入的作用。然而，这种作用在小鼠中尚未得到阐明。小鼠胚胎中的 MHP 很可能与鸡胚胎中的 MHP 一样，提供了神经板弯曲的位点，并且 MHP 内神经上皮细胞的楔入提供了产生纵向中线沟槽所需的力，围绕该沟槽随后发生神经板折叠（即神经折叠抬高）。需要进一步的研究来更准确地定义这些角色。 © 1994 Wiley-Liss, Inc.
Despite a wealth of information about cell behaviors contributing to neurulation in chick embryos, similar behaviors in mouse embryos have yet to be well characterized. This study examines cell behaviors occurring during bending of the mouse neural plate, in particular, qualitative and quantitative changes in neuroepithelial cell shape. Our current results demonstrate that in mouse embryos (1) the median hinge point (MHP), a localized region of neural plate that becomes anchored to the underlying prechordal plate mesoderm or notochord/ notochordal plate and forms a midline longitudinal furrow around which folding of the remaining neural plate (i.e., the part of the neural plate not involved in MHP formation) occurs, develops during stages of neural fold elevation; (2) the MHP is enriched with wedge‐shaped neuroepithelial cells but has significantly fewer spindle‐shaped, inverted wedge‐shaped, and globular neuroepithelial cells than do the adjacent paired lateral areas of the neuroepithelium (L); and (3) each L is enriched with spindle‐shaped, inverted wedge‐shaped, and globular neuroepithelial cells but has significantly fewer wedge‐shaped neuroepithelial cells than does the MHP. Thus wedging of neuroepithelial cells occurs during bending of the mouse neural plate and is localized to the MHP during neural fold elevation. Similarly, previous studies in the chick have shown that neuroepithelial cells become wedge shaped during bending of the neural plate and that such cell wedging is localized to the MHP during neural fold elevation. Such studies also have shed light on the roles of MHP formation and localized wedging of neuroepithelial cells within the MHP in the chick; however, such roles have yet to be elucidated in the mouse. It is probable that the MHP in mouse embryos, like that in chick embryos, provides a locus for bending of the neural plate and that wedging of neuroepithelial cells within the MHP provides the force necessary to generate the longitudinal midline furrow around which subsequent folding of the neural plate (i.e., neural fold elevation) occurs. Further studies are necessary to define these roles more precisely. © 1994 Wiley‐Liss, Inc.