Search strategy is regulated by somatostatin signaling and deep brain photoreceptors in zebrafish.
Search strategy is regulated by somatostatin signaling and deep brain photoreceptors in zebrafish.
复制标题
搜索策略受斑马鱼中的生长抑素信号传导和深脑感光体调节。
DOI：
10.1186/s12915-016-0346-2
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发表时间：
2017-01-26
期刊：
影响因子：
5.4
通讯作者：
Burgess HA
中科院分区：
文献类型：
--
作者：
Horstick EJ;Bayleyen Y;Sinclair JL;Burgess HA
动物使用感官线索来有效地定位资源，但当感官信息不足时，它们可能依赖于内部编码的搜索策略。尽管搜索行为的重要性，但对脊椎动物潜在的神经机制的理解有限。在这里，我们报告说，照明的损失启动复杂的光搜索行为的斑马鱼幼虫。使用三维跟踪，我们表明，在黑暗的发病幼虫游泳在一个螺旋形的轨迹，空间上限制在水平面上，然后逐渐过渡到一个向外运动的配置文件。本地和向外游泳模式显示区域限制和漫游搜索策略的特征，差异增强趋光性附近和远程光源。视网膜信号仅需要启动区域限制搜索，这意味着大脑内的光感受器驱动过渡到漫游搜索状态。支持这一点，orthopediaA突变幼虫表现出受损的过渡到漫游搜索，这是一种表型，这是概括的损失的非视觉视蛋白opn 4a和生长抑素信号。这些发现为区域限制和漫游搜索行为定义了不同的神经通路，并阐明了内部驱动如何促进目标导向的活动。本文的在线版本（doi：10.1186/s12915-016-0346-2）包含补充材料，可供授权用户使用。
Animals use sensory cues to efficiently locate resources, but when sensory information is insufficient, they may rely on internally coded search strategies. Despite the importance of search behavior, there is limited understanding of the underlying neural mechanisms in vertebrates. Here, we report that loss of illumination initiates sophisticated light-search behavior in larval zebrafish. Using three-dimensional tracking, we show that at the onset of darkness larvae swim in a helical trajectory that is spatially restricted in the horizontal plane, before gradually transitioning to an outward movement profile. Local and outward swim patterns display characteristic features of area-restricted and roaming search strategies, differentially enhancing phototaxis to nearby and remote sources of light. Retinal signaling is only required to initiate area-restricted search, implying that photoreceptors within the brain drive the transition to the roaming search state. Supporting this, orthopediaA mutant larvae manifest impaired transition to roaming search, a phenotype which is recapitulated by loss of the non-visual opsin opn4a and somatostatin signaling. These findings define distinct neuronal pathways for area-restricted and roaming search behaviors and clarify how internal drives promote goal-directed activity. The online version of this article (doi:10.1186/s12915-016-0346-2) contains supplementary material, which is available to authorized users.