A spectrophotometric assay for chlorine-containing compounds.
A spectrophotometric assay for chlorine-containing compounds.
复制标题
含氯化合物的分光光度测定。
DOI：
10.1016/0003-2697(91)90463-4
复制
发表时间：
1991
影响因子：
2.9
通讯作者：
Eaton,JW
中科院分区：
文献类型：
--
作者：
Chesney,JA;MahoneyJr,JR;Eaton,JW
次氯酸和氯胺化合物的测定在许多领域都很重要。现在有几种技术可用于此类分析，但大多数需要不稳定的试剂和/或分析过程中的多个步骤。我们已经开发了一种简单的，一步分光光度法测定反应性含氯化合物，包括抗坏血酸氧化次氯酸或氯胺。其他非卤化物氧化剂如过氧化氢或次硫氰酸不会干扰测定。由于少量的抗坏血酸不会破坏生物材料，这种方法还允许连续测量活化的中性粒细胞产生的含氯化合物。这种简单的分析方法可以精确分析低至1 nmol的HOCl。
Determinations of hypochlorous acid and chloramine compounds are important in a number of areas. Several techniques are now available for such analyses, but most require unstable reagents and/or multiple steps in the analytical procedure. We have developed a simple, one-step spectrophotometric assay for reactive chlorine-containing compounds involving the oxidation of ascorbic acid by hypochlorous acid or chloramines. There is no interference from other nonhalide oxidants such as hydrogen peroxide or hypothiocyanous acid. Because small amounts of ascorbic acid will not damage biological materials, this method also allows continuous measurements of the generation of chlorine-containing compounds by activated neutrophils. This simple assay permits precise analysis of as little as 1 nmol of HOCl.