Molecular survey of Babesia microti, Ehrlichia species and Candidatus Neoehrlichia mikurensis in wild rodents from Shimane Prefecture, Japan
Molecular survey of Babesia microti, Ehrlichia species and Candidatus Neoehrlichia mikurensis in wild rodents from Shimane Prefecture, Japan
复制标题
DOI：
10.1111/j.1348-0421.2007.tb03923.x
复制
发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Tsuji, Masayoshi
中科院分区：
文献类型：
--
作者：
Tabara, Kenji;Arai, Satoru;Tsuji, Masayoshi
在蜱传疾病流行的日本岛根县，大量患者被诊断为“不明原因发热”（FUO）。我们对来自日本岛根县的62例FUO病例和62例野生啮齿动物进行了田鼠巴氏杆菌、埃立克体属和三疣猴Neoehrlichia mikurensis的分子调查。PCR使用特异性引物的Babelias 18 S小亚基rRNA（rDNA）基因和Anaplasmataceae groESL扩增产物分别从45%（28/62）和25.8%（16/62）的捕获小鼠。在18 S rDNA PCR阳性的28份样品中，23份和5份样品为Hobetsu和Kobe B型阳性。Microti，分别。相比之下，在16份groESL PCR阳性样品中，8份、1份和7份样品对鼠埃立克体、埃立克体属HF 565和猪链球菌呈阳性。mikurensis，分别。将选定的血液样本接种到金色叙利亚仓鼠中，表明存在Hobetsu和Kobe B型。分别在四个和一个样品中发现了microti。后一种菌株的分离被认为是重要的，因为以前的研究表明，这种类型的分布迄今仅限于兵库县的淡路岛，第一例输血相关的人类巴贝虫病起源于那里。62例FUO人类病例的DNA样本检测为B阴性。PCR检测了田鼠18 SrDNA基因、无形体科groESL基因、日本立克次体17 K属共同抗原基因和恙虫病东方体56 K抗原基因。我们还对从岛根县收集的疑似携带蜱传疾病的FUO患者的62份血清进行了血清流行病学调查。然而，使用B. microti和E.小鼠感染细胞检测到抗E.只有一个阳性样本中有鼠。这项研究表明，在岛根县存在几个潜在的重要蜱传病原体，并建议有必要进一步研究的病原体的FUO。
A significant number of patients are diagnosed with "fevers of unknown origin" (FUO) in Shimane Prefecture in Japan where tick-borne diseases are endemic. We conducted molecular surveys for Babesia microti, Ehrlichia species, and Candidatus Neoehrlichia mikurensis in 62 FUO cases and 62 wild rodents from Shimane Prefecture, Japan. PCR using primers specific for the Babesia 18S small-subunit rRNA (rDNA) gene and Anaplasmataceae groESL amplified products from 45% (28/62) and 25.8% (16/62) of captured mice, respectively. Of the 28 18S rDNA PCR positives, 23 and five samples were positive for Hobetsu- and Kobe-type B. microti, respectively. In contrast, of the 16 groESL PCR positives, eight, one and seven samples were positive for Ehrlichia muris, Ehrlichia sp. HF565 and Candidatus N. mikurensis, respectively. Inoculation of selected blood samples into Golden Syrian hamsters indicated the presence of Hobetsu- and Kobe-type B. microti in four and one sample, respectively. Isolation of the latter strain was considered important as previous studies suggested that the distribution of this type was so far confined to Awaji Island in Hyogo Prefecture, where the first case of transfusion-associated human babesiosis originated. DNA samples from 62 FUO human cases tested negative for B. microti 18S rDNA gene, Anaplasmataceae groESL gene, Rickettsia japonica 17K genus-common antigen gene and Orientia tsutsugamushi 56K antigen gene by PCRs. We also conducted seroepidemiological surveys on 62 human sera collected in Shimane Prefecture from the FUO patients who were suspected of carrying tick-borne diseases. However, indirect immunofluorescent antibody tests using B. microti- and E. muris-infected cells detected IgG against E. muris in only a single positive sample. This study demonstrates the presence of several potentially important tick-borne pathogens in Shimane Prefecture and suggests the need for further study on the causative agents of FUOs.