31P and two-dimensional 31P/1H correlated NMR spectra of Duplex d(Ap[17O]Gp[18O]Cp[16O]T) and assignment of 31P signals in d(ApGpCpT)2-actinomycin D complex.
31P and two-dimensional 31P/1H correlated NMR spectra of Duplex d(Ap[17O]Gp[18O]Cp[16O]T) and assignment of 31P signals in d(ApGpCpT)2-actinomycin D complex.
复制标题
双链体 d(Ap[17O]Gp[18O]Cp[16O]T) 的 31P 和二维 31P/1H 相关 NMR 谱以及 d(ApGpCpT)2-放线菌素 D 复合物中 31P 信号的分配。
DOI：
10.1021/bi00321a067
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Shah,DO
中科院分区：
文献类型：
--
作者：
Gorenstein,DG;Lai,K;Shah,DO
大卫G. Gorenstein，* Kofen Lai，and Dinesh O. Shah摘要：固相亚磷酰胺法用于合成未标记和磷酰基标记的d（Ap-[170] Gp [180] Cp [160] T）。31标记d（ApGpCpT）的磷酰氧并由此分配31 P信号的能力，结合二维31 P/′ H化学位移相关NMR光谱技术，提供了一种新的手段。实际上，这种药物和相关药物的大部分药理学活性源自它们在核酸的堆叠碱基对之间的直接嵌入（Lown，1977）。当DNA结构受到干扰时，这些药物可能会抑制DNA结构。
David G. Gorenstein,* Kofen Lai, and Dinesh O. Shah abstract: A solid-phase phosphoramidite method was used for the synthesis of unlabeled and phosphoryl-labeled d (Ap-[170] Gp [180] Cp [160] T). The ability to label the phosphoryl oxygens of d (ApGpCpT) and thus assign the 31P signals, combined with a two-dimensional 31P/'H chemical shift correlated NMR spectral technique, provided a novel meansThe interaction of drugs such as the antitumor antibiotic actinomycin D (Act D) 1 with nucleic acids is believed to perturb the conformation of the sugar-phosphate backbone. Indeed, much of the pharmacological activity of this and re-lated drugs derives from their direct intercalation between stacked base pairs of the nucleic acids (Lown, 1977). When DNA structure is perturbed, these drugs may inhibit the