A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
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DOI：
10.1038/srep41585
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发表时间：
2017-01-31
影响因子：
4.6
通讯作者：
Tempest，Helen G.
中科院分区：
文献类型：
--
作者：
Ioannou，Dimitrios;Millan，Nicole M.;Tempest，Helen G.
精子核中染色体的组织被认为具有独特的发夹-环状排列，这种排列被认为有助于受精后父亲基因组的有序外流。这项研究同时评估了端粒、着丝粒的3D和2D径向和纵向组织，并调查了染色体是否在精子细胞中形成了相同的着丝粒簇。在多个受试者中，使用3D和2D方法观察到所有被调查基因座的可重复的径向和纵向非随机组织。我们报告了新的发现，端粒和着丝粒分布在整个细胞核中，但在核外围和中间区域显示出大约1：1的分布，其中15%占据核内部。端粒和着丝粒聚集在精子核中，平均形成20个和7个着丝粒。可复制的纵向组织显示端粒和着丝粒优先定位在精子细胞的中间区域。初步证据也支持这样的假设，即特定的染色体优先形成相同的着丝粒簇。本研究中所描述的端粒和着丝粒的节段性分布可以更容易地适应和促进受精后父亲染色体的连续外流。
The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized to aid in the ordered exodus of the paternal genome following fertilization. This study simultaneously assessed the 3D and 2D radial and longitudinal organization of telomeres, centromeres, and investigated whether chromosomes formed the same centromere clusters in sperm cells. Reproducible radial and longitudinal non-random organization was observed for all investigated loci using both 3D and 2D approaches in multiple subjects. We report novel findings, with telomeres and centromeres being localized throughout the nucleus but demonstrating roughly a 1:1 distribution in the nuclear periphery and the intermediate regions with <15% occupying the nuclear interior. Telomeres and centromeres were observed to aggregate in sperm nuclei, forming an average of 20 and 7 clusters, respectively. Reproducible longitudinal organization demonstrated preferential localization of telomeres and centromeres in the mid region of the sperm cell. Preliminary evidence is also provided to support the hypothesis that specific chromosomes preferentially form the same centromere clusters. The more segmental distribution of telomeres and centromeres as described in this study could more readily accommodate and facilitate the sequential exodus of paternal chromosomes following fertilization.