Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level
Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level
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DOI：
10.1093/carcin/bgab025
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发表时间：
2021-03-22
期刊：
影响因子：
4.7
通讯作者：
Taketomi, Akinobu
中科院分区：
文献类型：
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作者：
Ohira, Masafumi;Yokoo, Hideki;Taketomi, Akinobu
脂肪酸结合蛋白5（FABP5）在肝细胞癌（HCC）组织中高度表达，并与HCC进展相关。在这项研究中，我们分析了血清FABP5（sFABP5）作为肝癌肿瘤标志物的潜力及其在肝癌进展中的临床意义。我们比较了HCC患者（HCC组）和无HCC的肝炎患者（肝炎组）的sFABP5浓度。此外，我们还检测了肝癌切除组织中FABP5的表达水平（tFABP5），并分析了它们与sFABP5的关系。我们还使用FABP5敲除和过表达HCC细胞系进行了基于细胞的测定，以分析外源性FABP5（exFABP5）对HCC细胞的影响。我们发现sFABP5不是HCC的有用肿瘤标志物，因为HCC和sFABP5不相关。然而，sFABP5和tFABP5与HCC术后生存率显著相关，而sFABP5和tFABP5彼此独立。在基于细胞的测定中，exFABP5被HCC细胞系摄取，并通过补充内源性FABP5功能积极影响葡萄糖耗尽条件下的细胞存活。总之，sFABP5通过在葡萄糖耗尽条件下增加细胞活力而对HCC进展具有显著影响，而与tFABP5无关。由于tFABP5和5FABP5是HCC进展中相互独立的重要因素，因此在改善HCC患者的预后时应将两者独立考虑。
Fatty acid-binding protein 5 (FABP5) is highly expressed in hepatocellular carcinoma (HCC) tissues and is related to HCC progression. In this study, we analyzed the potential of serum FABP5 (sFABP5) as a tumor marker in HCC and its clinical significance in HCC progression. We compared the sFABP5 concentration in patients with HCC (HCC group) with that of patients with hepatitis without HCC (hepatitis group). Moreover, we measured the FABP5 expression levels in resected HCC tissues (tFABP5) and analyzed their relationship with sFABP5. We also performed cell-based assays using FABP5 knockout and overexpressing HCC cell lines to analyze the effect of extrinsic FABP5 (exFABP5) on HCC cells. We showed that sFABP5 was not a useful tumor marker for HCC, as HCC and sFABP5 were not correlated. However, sFABP5 and tFABP5 significantly correlated with survival after surgery for HCC, while sFABP5 and tFABP5 were independent of each other. In cell-based assays, exFABP5 was taken up by HCC cell lines and positively affected cell survival under glucose-depleted conditions by complementing the endogenous FABP5 function. In conclusion, sFABP5 had a significant impact on HCC progression irrespective of tFABP5 by augmenting cell viability under glucose-depleted conditions. As tFABP5 and 5FABP5 are important factors that are independent of each other in HCC progression, both of them should be considered independently in improving the prognosis of patients with HCC.