THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT
THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT
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DOI：
10.1101/gad.7.4.555
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发表时间：
1993-04-01
影响因子：
10.5
通讯作者：
ELLEDGE, SJ
中科院分区：
文献类型：
--
作者：
DURFEE, T;BECHERER, K;ELLEDGE, SJ
视网膜母细胞瘤蛋白（p110 RB）与许多细胞蛋白质相互作用，形成复合物，对它的生长抑制功能可能很重要。我们已经开发并使用了酵母双杂交系统的改进版本，以分离人类cDNA编码的蛋白质能够结合p110 RB。一个克隆编码一种新的1型蛋白磷酸酶催化亚基（PP-1alpha 2），它不同于最初定义的PP-1alpha的氨基末端11个氨基酸插入。体外结合试验表明，PP-1 α亚型优先结合低磷酸化形式的p110 RB。此外，结合PP-1 α 2和SV 40大T抗原需要相似的p110 RB序列。细胞周期同步性实验表明，这种关联发生在有丝分裂到G1早期。这两种蛋白质的调节这些研究结果的影响进行了讨论。
The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1alpha2), which differs from the originally defined PP-1alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1alpha2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed.