IDENTIFICATION OF CRYPTIC SITES OF DNA-SEQUENCE AMPLIFICATION IN HUMAN BREAST-CANCER BY CHROMOSOME MICRODISSECTION
IDENTIFICATION OF CRYPTIC SITES OF DNA-SEQUENCE AMPLIFICATION IN HUMAN BREAST-CANCER BY CHROMOSOME MICRODISSECTION
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DOI：
10.1038/ng1094-155
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发表时间：
1994-10-01
期刊：
影响因子：
30.8
通讯作者：
TRENT, JM
中科院分区：
文献类型：
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作者：
GUAN, XY;MELTZER, PS;TRENT, JM
我们对来自 9 种不同乳腺癌细胞系的 16 个假定的均匀染色区域 (hsrs) 进行了显微切割，以确定它们的染色体起源和组成。正如预期的那样，最常见的扩增染色体带区是 17q12（包含 ERBB2）。然而，也鉴定出了不包含已知癌基因的区域，包括13q31（5/9例）和20q12-13.2（4/9例）。确定了每个 hsr 内整合的扩增 DNA 的染色体组成，在 13/16 例 (81%) 中，hsr 显示由两个或多个染色体区域组成。这些研究揭示了 hsrs 的形成机制，并确定了可能含有乳腺癌扩增基因的染色体区域。
We have performed microdissection of 16 putative homogeneously staining regions (hsrs) from nine different breast cancer cell lines in order to determine their chromosomal origin and composition. As expected, the most commonly amplified chromosomal band-region was 17q12 (containing ERBB2). However, regions not containing known oncogenes were also identified, including 13q31 (5/9 cases) and 20q12-13.2 (4/9 cases). The chromosomal composition of the integrated amplified DNA within each hsr was determined and in 13/16 cases (81%), hsrs were shown to be composed of two or more chromosomal regions. These studies shed light on the mechanism of formation of hsrs, and identify chromosomal regions likely to harbour genes amplified in breast cancer.