DNA double-strand breaks as a method of radiation measurements for therapeutic beams.
DNA double-strand breaks as a method of radiation measurements for therapeutic beams.
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DNA双链破裂是治疗束的辐射测量方法。
DOI：
10.1002/mp.12956
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发表时间：
2018-07
期刊：
影响因子：
3.8
通讯作者：
Kirby N
中科院分区：
文献类型：
--
作者：
Obeidat M;McConnell KA;Li X;Bui B;Stathakis S;Papanikolaou N;Rasmussen K;Ha CS;Lee SE;Shim EY;Kirby N
许多类型的剂量计用于测量辐射剂量和校准放射治疗设备，但没有直接测量该剂量的生物效应。本文的目的是建立一个剂量计，可以测量DNA的双链断裂（DSB）的概率，这是直接相关的辐射的生物效应。将DNA剂量计悬浮在磷酸盐缓冲盐水（PBS）中，所述DNA剂量计由磁性链霉亲和素珠组成，所述磁性链霉亲和素珠连接到在相对端上用生物素和荧光素酰胺（FAM）标记的四个双链酶对DNA链。将50微升样品置于水箱装置内的塑料管中，并以25、50、100、150和200戈伊的剂量水平进行辐照。辐照后，使用磁铁将剂量计机械分离成珠（完整DNA）和上清液（断裂DNA/FAM）。读取荧光并计算DSB的概率。该DNA剂量计响应以Southern印迹分析技术为基准，用于测量DSB概率。对于DNA剂量计，在25、50、100、150和200戈伊剂量水平下的DSB概率分别为0.043、0.081、0.149、0.196和0.242，平均值的标准误差分别为0.002、0.003、0.006、0.005和0.011。对于Southern印迹法，在25、50、100、150和200戈伊剂量水平下DSB的概率分别为0.053、0.105、0.198、0.235和0.264，平均值的标准误差分别为0.013、0.024、0.040、0.044和0.063。DNA剂量计可以准确地确定DNA双链断裂（DSB）的概率，这是放射治疗的最大毒性效应之一，吸收的辐射剂量为25至200戈伊。这是证明DNA剂量计作为辐射测量技术的可行性的重要一步。
Many types of dosimeters are used to measure radiation dose and calibrate radiotherapy equipment, but none directly measure the biological effect of this dose. The purpose here is to create a dosimeter that can measure the probability of double-strand breaks (DSB) for DNA, which is directly related to the biological effect of radiation. A DNA dosimeter, consisting of magnetic streptavidin beads attached to four kilobase pair DNA strands labeled with biotin and fluorescein amidite (FAM) on opposing ends, was suspended in phosphate-buffered saline (PBS). Fifty microliter samples were placed in plastic tubes inside a water tank setup and irradiated at the dose levels of 25, 50, 100, 150, and 200 Gy. After irradiation, the dosimeters were mechanically separated into beads (intact DNA) and supernatant (broken DNA/FAM) using a magnet. The fluorescence was read and the probability of DSB was calculated. This DNA dosimeter response was benchmarked against a Southern blot analysis technique for the measurement of DSB probability. For the DNA dosimeter, the probabilities of DSB at the dose levels of 25, 50, 100, 150, and 200 Gy were 0.043, 0.081, 0.149, 0.196, and 0.242, respectively, and the standard errors of the mean were 0.002, 0.003, 0.006, 0.005, and 0.011, respectively. For the Southern blot method, the probabilities of DSB at the dose levels of 25, 50, 100, 150, and 200 Gy were 0.053, 0.105, 0.198, 0.235, and 0.264, respectively, and the standard errors of the mean were 0.013, 0.024, 0.040, 0.044, and 0.063, respectively. A DNA dosimeter can accurately determine the probability of DNA double-strand break (DSB), one of the most toxic effects of radiotherapy, for absorbed radiation doses from 25 to 200 Gy. This is an important step in demonstrating the viability of DNA dosimeters as a measurement technique for radiation.
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DOI：
10.1016/j.mrgentox.2006.03.002
发表时间：
2006-06-16
影响因子：
1.9
作者：
Kumaravel, T. S.;Jha, Awadhesh N.
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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