Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome
Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome
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DOI：
10.1016/0092-8674(95)90136-1
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发表时间：
1995-12-29
期刊：
影响因子：
64.5
通讯作者：
Maniatis, T
中科院分区：
文献类型：
--
作者：
Thanos, D;Maniatis, T
我们目前的证据表明，人干扰素β（IFN β）基因的转录激活需要组装的高阶转录增强子复合物（增强体）。这种多组分复合物包括至少三种不同的转录因子和高迁移率族蛋白HMG I（Y）。这种复合物的体外组装和体内转录活性都需要单个转录因子结合位点之间的精确螺旋关系。此外，HMG I（Y）特异性结合增强子内的三个位点，促进体外转录因子的协同结合，并且是这些因子之间体内转录协同作用所需的。因此，HMG I（Y）在IFN β基因增强体的组装和功能中起重要作用。
We present evidence that transcriptional activation of the human interferon-beta (IFN beta) gene requires the assembly of a higher order transcription enhancer complex (enhanceosome). This multicomponent complex includes at least three distinct transcription factors and the high mobility group protein HMG I(Y). Both the in vitro assembly and in vivo transcriptional activity of this complex require a precise helical relationship between individual transcription factor-binding sites. In addition, HMG I(Y), which binds specifically to three sites within the enhancer, promotes cooperative binding of transcription factors in vitro and is required for transcriptional synergy between these factors in vivo. Thus, HMG I(Y) plays an essential role in the assembly and function of the IFN beta gene enhanceosome.