Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14
Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14
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DOI：
10.1261/rna.2053710
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发表时间：
2010-06-01
期刊：
影响因子：
4.5
通讯作者：
Buehler, Marc
中科院分区：
文献类型：
--
作者：
Keller, Claudia;Woolcock, Katrina;Buehler, Marc
裂殖酵母Cid 14蛋白属于一个非典型的poly（A）聚合酶家族，其涉及广泛的生物学功能。在这里，我们描述了一个广泛的Cid 14蛋白质-蛋白质相互作用网络及其生化解剖。Cid 14与锌指蛋白Air 1最稳定地相互作用以形成Cid 14-Air 1复合物（CAC）。Cid 14与60 S核糖体亚基沉淀并与60 S组装因子共纯化，从而提供了与核糖体RNA加工的联系。与此相反，没有物理联系染色质已被确定，虽然基因表达谱显示，有效沉默的一些异染色质基因依赖于Cid 14和/或空气1。
The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in a broad range of biological functions. Here we describe an extensive Cid14 protein-protein interaction network and its biochemical dissection. Cid14 most stably interacts with the zinc-knuckle protein Air1 to form the Cid14-Air1 complex (CAC). Providing a link to ribosomal RNA processing, Cid14 sediments with 60S ribosomal subunits and copurifies with 60S assembly factors. In contrast, no physical link to chromatin has been identified, although gene expression profiling revealed that efficient silencing of a few heterochromatic genes depends on Cid14 and/or Air1.