Intraperitoneal injection of chloral hydrate causes intra-abdominal adhesions and unilateral testicular atrophy in golden Syrian hamsters.
Intraperitoneal injection of chloral hydrate causes intra-abdominal adhesions and unilateral testicular atrophy in golden Syrian hamsters.
复制标题
腹腔注射水合氯醛导致金色叙利亚仓鼠腹腔粘连和单侧睾丸萎缩。
DOI：
10.1016/0024-3205(92)90215-b
复制
发表时间：
1992
期刊：
影响因子：
6.1
通讯作者：
Blake,CA
中科院分区：
文献类型：
--
作者：
Dada,MO;Campbell,GT;Horacek,MJ;Blake,CA
我们调查了我们观察到的用水合氯醛（CH）腹腔注射麻醉的金色叙利亚仓鼠的高死亡率的原因。将完整的雄性叙利亚金仓鼠腹腔注射0.1cc/100g BW的35% CH溶液、35%氯化钠溶液或双蒸馏水。每组中等量的仓鼠在腹部右侧或左侧注射。在10天内，注射ch的仓鼠中有35%死亡或被安乐死。尸检显示严重的腹膜炎和动态肠梗阻。注射了ch的仓鼠体重增加的速度与对照组相似。所有幸存的仓鼠在注射后18天被杀死。在注射ch的存活仓鼠中，84.6%的仓鼠出现腹腔粘连，61.5%的仓鼠出现单侧睾丸萎缩，53.8%的仓鼠出现附睾脂肪垫（EFP）淡黄色坏死块。所有病变都发生在注射一侧。由于腹内粘连，萎缩的睾丸呈隐睾状。在经过水处理的对照组中，没有出现异常；而在生理盐水对照组中，75%的EFP有肿块。注射CH或高渗盐水的仓鼠EFP肿块的组织学相似，提示脂肪坏死的诊断。结果提示，单次腹腔注射CH可引起死亡、腹腔粘连和单侧隐睾，而EFP脂肪坏死可能是由高浓度盐引起的。结果进一步表明，在慢性研究中，特别是男性生殖系统的研究中，不应通过腹腔注射高浓度的CH进行麻醉。
We investigated the reason for the high mortality we had observed in hypophysectomized-orchidectomized Golden Syrian hamsters that were anesthesized with intraperitoneal (i.p.) injections of chloral hydrate (CH). Intact male Golden Syrian hamsters were injected intraperitoneally with 0.1cc/100g BW of a 35% solution of CH, a 35% solution of sodium chloride, or double-distilled water. Equal numbers of hamsters in each group were injected on the right or left side of the abdomen. Within 10 days, 35% of the CH-injected hamsters were dead or had to be euthanized. Autopsy revealed severe peritonitis and adynamic ileus. CH-injected hamsters that survived gained weight at a rate similar to that of the controls. All surviving hamsters were killed 18 days after the injections. Among the surviving CH-injected hamsters, 84.6% had intra-abdominal adhesions, 61.5% had unilateral testicular atrophy, and 53.8% had a yellowish necrotic mass in the epididymal fat pad (EFP). All the lesions occured on the side that was injected. The atrophied testes had been rendered cryptorchid due to involvement with intra-abdominal adhesions. In the water-treated controls, there were no abnormalities; whereas, in the saline controls, 75% had a mass in the EFP. Histology of the EFP mass was similar in hamsters injected with CH or hypertonic saline and suggested a diagnosis of fat necrosis. The results suggest that the mortality, the intra-abdominal adhesions, and the unilateral cryptorchidism were caused by a single i.p. injection of CH, but the fat necrosis in the EFP was probably caused by high concentrations of salt. The results further suggest that high concentrations of CH should not be injected intraperitoneally for anesthesia in chronic studies, particularly of the male reproductive system.