The topographic specificity of muscle reinnervation predicts function.
The topographic specificity of muscle reinnervation predicts function.
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肌肉神经支配的地形特异性可预测功能。
DOI：
10.1111/ejn.13058
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Brushart,Thomas
中科院分区：
文献类型：
--
作者：
O'Daly,Andres;Rohde,Charles;Brushart,Thomas
功能测试在验证实验性神经修复的成功方面已经逐渐占据主导地位。然而，在一个模型中获得的结果不能预测其他模型的结果，因为它们反映了多个关节上几块肌肉的协调相互作用。因此，地形正确和不正确的肌肉神经再支配的许多组合可以产生相同的结果。我们已经开发了一个二元模型，其中肘屈肌和伸肌被再神经支配，肘关节屈曲和伸展是测试的功能。对Lister-Hooded大鼠的肌皮神经和桡神经进行轴突损伤，在损伤部位产生越来越大程度的轴突错误方向，从简单的挤压到近端和远端残端的横断和旋转偏移。肘部功能用一种装置进行测试，该装置需要协调的肘部伸展以达到糖丸和弯曲以将它们返回到口中。再生12周后，用WGA-Ruby逆行标记投射到远端肌皮神经的运动神经元，并对其在肌皮或桡神经运动神经元池中的位置进行评分。初次手术导致的轴突错误方向的严重程度反映了桡神经轴突对肌皮神经的不适当再支配的进展程度。神经再支配的特异性预测肘关节功能（r= 0.72），而运动神经元再生的数量没有。因此，该模型非常适合研究单个关节的再生特异性和功能的相互作用，并产生比从更复杂的模型获得的数据更广泛的数据。
Functional testing has assumed a progressively dominant role in validating the success of experimental nerve repair. Results obtained in one model, however, cannot predict the results in others because they reflect the coordinated interaction of several muscles across multiple joints. As a result, many combinations of topographically correct and incorrect muscle reinnervation could produce the same result. We have developed a binary model in which elbow flexors and extensors are reinnervated, and elbow flexion and extension are the functions tested. The musculocutaneous and radial nerves of Lister‐Hooded rats were subjected to axonotmetic injuries that produced increasing degrees of axonal misdirection at the site of injury ranging from simple crush to transection and rotational offset of proximal and distal stumps. Elbow function was tested with a device that requires coordinated elbow extension to reach sugar pellets and flexion to return them to the mouth. After 12 weeks of regeneration, motoneurons projecting to the distal musculocutaneous nerve were retrogradely labelled with WGA‐Ruby and scored regarding their location within musculocutaneous or radial motoneuron pools. The severity of axonal misdirection resulting from the initial surgery was mirrored by progressive degrees of inappropriate reinnervation of the musculocutaneous nerve by radial nerve axons. The specificity of reinnervation predicted elbow function (r= 0.72), whereas the number of motoneurons regenerating did not. This model is thus well suited to study the interaction of regeneration specificity and function across a single joint, and to produce data that can be generalized more broadly than those obtained from more complex models.