mRatBN7.2: familiar and unfamiliar features of a new rat genome reference assembly.
mRatBN7.2: familiar and unfamiliar features of a new rat genome reference assembly.
复制标题
mRatBN7.2：新的大鼠基因组参考组件的熟悉和不熟悉的特征。
DOI：
10.1152/physiolgenomics.00017.2022
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发表时间：
2022
影响因子：
4.6
通讯作者：
Doris,PeterA
中科院分区：
文献类型：
--
作者：
deJong,TristanV;Chen,Hao;Brashear,WesleyA;Kochan,KelliJ;Hillhouse,AndrewE;Zhu,Yaming;Dhande,IshaS;Hudson,ElizabethA;Sumlut,MaryH;Smith,MelissaL;Kalbfleisch,TheodoreS;Doris,PeterA
与人类和小鼠相比，大鼠基因组工具的出现速度较慢，并且仍然不那么彻底和全面。2020年底新的改进的大鼠参考基因组mRatBN 7.2的到来是一个值得欢迎的事件。该组装体与前代大鼠参考组装体一样，来源于近交系Brown Norway大鼠。在这个“用户”调查中，我们希望为这个程序集的其他用户提供一些关于它的特性的见解和一些关于它的改进的评估，以及从这个程序集的独特方面产生的一些警告。mRatBN 7.2由Wellcome桑格研究所作为大型脊椎动物基因组计划的一部分产生。这种大鼠组合现在已经加入了国家生物技术信息中心（NCBI）基因组参考联盟的人类，小鼠，鸡和斑马鱼，该联盟提供了对该组合的持续管理。在这里，我们检查的技术程序，该程序集的创建和评估如何构成一个改进，它的前身。我们还指出了影响组件的技术限制，并提供了这些限制如何产生的插图以及对该参考组件的影响。
Rat genomic tools have been slower to emerge than for those of humans and mice and have remained less thorough and comprehensive. The arrival of a new and improved rat reference genome, mRatBN7.2, in late 2020 is a welcome event. This assembly, like predecessor rat reference assemblies, is derived from an inbred Brown Norway rat. In this “user” survey we hope to provide other users of this assembly some insight into its characteristics and some assessment of its improvements as well as a few caveats that arise from the unique aspects of this assembly. mRatBN7.2 was generated by the Wellcome Sanger Institute as part of the large Vertebrate Genomes Project. This rat assembly has now joined human, mouse, chicken, and zebrafish in the National Center for Biotechnology Information (NCBI)’s Genome Reference Consortium, which provides ongoing curation of the assembly. Here we examine the technical procedures by which the assembly was created and assess how this assembly constitutes an improvement over its predecessor. We also indicate the technical limitations affecting the assembly, providing illustrations of how these limitations arise and the impact that results for this reference assembly.