Silibinin Inhibits Adipogenesis and Induces Apoptosis in 3T3-L1 Adipocytes
Silibinin Inhibits Adipogenesis and Induces Apoptosis in 3T3-L1 Adipocytes
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DOI：
10.4014/mbl.1610.10005
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发表时间：
2017-03-01
影响因子：
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通讯作者：
Nam, Ju-Ock
中科院分区：
文献类型：
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作者：
Lee, Seul Gi;Kwon, Taeg Kyu;Nam, Ju-Ock
C/EBP β和C/EBPd是启动脂肪形成所必需的，并诱导关键脂肪形成调节因子如PPAR的表达。C/EBPa。在本研究中，我们研究了水飞蓟宾在调节脂肪细胞分化和C/EBP β和C/EBPd在脂肪形成的早期阶段的表达的作用及其可能的分子机制。水飞蓟宾在统计学上显著抑制细胞内脂质积聚和脂肪形成过程中不同阶段所涉及的各种基因的mRNA表达。水飞蓟宾还抑制3 T3-L1脂肪细胞中脂蛋白脂酶（LPL）、脂肪酸结合蛋白4（AP 2）和脂联素的表达。因此，水飞蓟宾的抗脂肪形成作用似乎源于抑制C/EBP β和C/EBPd表达的能力。此外，水飞蓟宾降低分化期的细胞活力，并通过capspase-3激活诱导凋亡性细胞死亡。
C/EBP beta and C/EBPd are required for the initiation of adipogenesis and induce the expression of key adipogenic regulators, such as PPAR. and C/EBPa. In the present study, we have examined the effects of silibinin and its possible molecular mechanisms in regulating adipocyte differentiation and expression of C/EBP beta and C/EBPd in the early stage of adipogenesis. Silibinin statistically significantly inhibits intracellular lipid accumulation and the mRNA expression of various genes involved at different stages during adipogenesis. Silibinin also suppresses expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (AP2), and adiponectin in 3T3-L1 adipocytes. Thus, the anti-adipogenic effect of silibinin seems to originate from the ability to inhibit the expression of C/EBP beta and C/EBPd. Furthermore, silibinin decreases cell viability for differentiation period and induces apoptotic cell death through capspase-3 activation.