Escherichia coli K Bacteriophages I. Isolation and Introductory Characterization of Five Escherichia coli K Bacteriophages
Escherichia coli K Bacteriophages I. Isolation and Introductory Characterization of Five Escherichia coli K Bacteriophages
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大肠杆菌 K 噬菌体 I. 五种大肠杆菌 K 噬菌体的分离和介绍性表征
DOI：
10.1128/jvi.2.10.1107-1114.1968
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发表时间：
1968
影响因子：
5.4
通讯作者：
S. Stirm
中科院分区：
文献类型：
--
作者：
S. Stirm
已分离出一组五种大肠杆菌K.这些细菌被吸附并溶解宿主细菌的荚膜形式，而其自发的无荚膜突变体不受影响。所有宿主菌株均为E. coli K抗原的热稳定A型，它们容易分离无囊突变体。在四个噬菌体-宿主系统中，发现获得的所有次级生长都是无囊的。对38株E.大肠杆菌和克雷伯氏菌的突变株均小于1个/105空斑形成单位，用该菌株免疫家兔，未产生交叉中和抗体。潜伏期（16和30分钟之间）和平均爆发大小（145和580之间），通过一步生长实验确定。
A set of five Escherichia coli K phages has been isolated. These phages are adsorbed to and lyse the capsular forms of the host bacteria, whereas their spontaneous, acapsular mutants are not affected. All host strains are heavily encapsulated test strains for E. coli K antigens of the thermostable A type and they readily segregate acapsular mutants. In four of the phage-host systems, all secondary growth obtained was found to be acapsular. When tested for host-range mutants on 38 strains of E. coli and Klebsiella, less than one mutant per 105 plaque-forming units was found. No cross-reacting neutralizing antibodies were obtained when rabbits were immunized with the K phages. The latent periods (between 16 and 30 min) and average burst sizes (between 145 and 580) were determined by one-step growth experiments.