Novel graphene-based biosensor for early detection of Zika virus infection
Novel graphene-based biosensor for early detection of Zika virus infection
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DOI：
10.1016/j.bios.2017.08.051
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发表时间：
2018-02-15
影响因子：
12.6
通讯作者：
Goldsmith, Brett R.
中科院分区：
文献类型：
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作者：
Afsahi, Savannah;Lerner, Mitchell B.;Goldsmith, Brett R.
我们开发了一种具有成本效益的便携式石墨烯生物传感器，用高度特异性的固定化单克隆抗体检测寨卡病毒。利用与石墨烯共价连接的单克隆抗体进行场效应生物传感（FEB），可以实时、定量地检测原生寨卡病毒（ZIKV）抗原。在低至450 pM的缓冲液中检测到的抗原剂量（ZIKV NS1）反应的电容百分比变化与临床意义水平一致。通过测量模拟人血清中的寨卡病毒抗原，证明了潜在的诊断应用。用日本脑炎NS1（一种同源且具有潜在交叉反应的病毒抗原）验证了选择性。此外，石墨烯平台可以同时提供非临床生物物理动力学工具的先进定量数据，使其适用于临床研究和可能的诊断应用。这种石墨烯驱动的寨卡生物传感器的速度、灵敏度和选择性使其成为医学诊断测试的理想候选者。
We have developed a cost-effective and portable graphene-enabled biosensor to detect Zika virus with a highly specific immobilized monoclonal antibody. Field Effect Biosensing (FEB) with monoclonal antibodies covalently linked to graphene enables real-time, quantitative detection of native Zika viral (ZIKV) antigens. The percent change in capacitance in response to doses of antigen (ZIKV NS1) coincides with levels of clinical significance with detection of antigen in buffer at concentrations as low as 450 pM. Potential diagnostic applications were demonstrated by measuring Zika antigen in a simulated human serum. Selectivity was validated using Japanese Encephalitis NS1, a homologous and potentially cross-reactive viral antigen. Further, the graphene platform can simultaneously provide the advanced quantitative data of nonclinical biophysical kinetics tools, making it adaptable to both clinical research and possible diagnostic applications. The speed, sensitivity, and selectivity of this first-of-its-kind graphene-enabled Zika biosensor make it an ideal candidate for development as a medical diagnostic test.