Brown Planthopper Nudivirus DNA Integrated in Its Host Genome
Brown Planthopper Nudivirus DNA Integrated in Its Host Genome
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DOI：
10.1128/jvi.03166-13
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发表时间：
2014-05-01
影响因子：
5.4
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
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作者：
Cheng, Ruo-Lin;Xi, Yu;Zhang, Chuan-Xi
褐飞虱（BPH），Nilaparvata lugens（半翅目：飞虱科），是亚洲水稻作物最具破坏性的害虫之一。在 BPH 的全基因组测序过程中发现了类似 Nudivirus 的序列。 PCR检查显示，从东亚、东南亚和南亚收集的所有22个BPH群体中都存在病毒序列。鉴定出 33 个裸病毒核心基因中的 32 个，其中包括 20 个杆状病毒核心基因的同源物。此外，在部分病毒基因组中还发现了几个与其他裸病毒共线排列的基因簇。在使用超矩阵方法构建的系统发育树中，原始病毒与其他裸病毒归为一组，并且与多DNA病毒密切相关。总而言之，这些数据表明该病毒序列属于裸病毒科的新成员。更具体地说，病毒序列在共同进化过程中被整合到昆虫宿主的染色体中。这项研究是首次报道吸食半翅目昆虫中的大型双链环状 DNA 病毒基因组。
The brown planthopper (BPH), Nilaparvata lugens (Hemiptera: Delphacidae), is one of the most destructive insect pests of rice crops in Asia. Nudivirus-like sequences were identified during the whole-genome sequencing of BPH. PCR examination showed that the virus sequences were present in all of the 22 BPH populations collected from East, Southeast, and South Asia. Thirty-two of the 33 nudivirus core genes were identified, including 20 homologues of baculovirus core genes. In addition, several gene clusters that were arranged collinearly with those of other nudiviruses were found in the partial virus genome. In a phylogenetic tree constructed using the supermatrix method, the original virus was grouped with other nudiviruses and was closely related to polydnavirus. Taken together, these data indicated that the virus sequences belong to a new member of the family Nudiviridae. More specifically, the virus sequences were integrated into the chromosome of its insect host during coevolution. This study is the first report of a large double-stranded circular DNA virus genome in a sap-sucking hemipteran insect.