Human cell lines that elaborate colon-stimulating activity for the marrow cells of man and other species.
Human cell lines that elaborate colon-stimulating activity for the marrow cells of man and other species.
复制标题
人类细胞系为人类和其他物种的骨髓细胞提供结肠刺激活性。
DOI：
10.1182/blood.v51.3.507.507
复制
发表时间：
1978
期刊：
影响因子：
20.3
通讯作者：
Lichtman
中科院分区：
文献类型：
--
作者：
John;F. Di;Persio;James;K.;Brennan;Marshall;A.;Lichtman
我们已经建立了两个人类细胞系，阐述了至少四个物种的集落刺激活性（CSA）：人，小鼠，兔和狗。一个是GCT，分离自纤维组织细胞瘤的肺转移;另一个是RC 4，分离自正常血液中富含单核细胞的部分。GCT或RC 4细胞条件培养基在体外刺激人骨髓生长方面比单核细胞条件培养基或人白细胞饲养层更有效。用Sephacryl S-200层析分离细胞系条件培养基表明，CSA对人骨髓细胞的最大活性在30，000 - 40，000道尔顿范围内洗脱。这些细胞系提供了用于纯化CSA的大量条件培养基的连续来源。此外，恒定的生长支持活动的所有物种的测试和细胞系CSA的高效力有利于其制定和生物学效应的研究。
We have established two human cell lines which elaborate colony-stimulating activity (CSA) for at least four species: man, mouse, rabbit, and dog. One, GCT, was isolated from a lung metastasis of a fibrous histiocytoma; the other, RC4, from a monocyte-enriched fraction of normal blood. Medium conditioned by either GCT or RC4 cells was more potent in stimulating human marrow growth in vitro than was monocyte-conditioned medium or human leukocyte feeder layers. Fractionation of cell-line-conditioned medium by Sephacryl S-200 chromatography indicated that the maximum activity of the CSA for human marrow cells is eluted within the range of 30,000-40,000 daltons. These cells lines provide a continuous source of large quantities of conditioned medium for purification of CSA. Moreover, the invariable growth-supporting activity for all species tested and the high potency of cell-line CSA facilitates studies of its elaboration and biologic effects.