Apoplastic recognition of multiple candidate effectors from the wheat pathogen Zymoseptoria tritici in the nonhost plant Nicotiana benthamiana.
Apoplastic recognition of multiple candidate effectors from the wheat pathogen Zymoseptoria tritici in the nonhost plant Nicotiana benthamiana.
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DOI：
10.1111/nph.14215
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Kanyuka K
中科院分区：
文献类型：
--
作者：
Kettles GJ;Bayon C;Canning G;Rudd JJ;Kanyuka K
真菌Zymoseptoria triumphia是小麦叶片的严格质外体、宿主特异性病原体，也是三孢壳针孢斑点病（STB）的病原体。所有其他植物被认为是非寄主，但非寄主抗性（NHR）的机制尚未解决之前。我们试图开发本氏烟草作为研究NHR对抗白叶枯病菌的系统。利用荧光显微镜和定量逆转录聚合酶链反应技术研究了玉米与本氏烟草之间的相互作用。使用农杆菌介导的瞬时表达来筛选本氏烟草中用于识别的推定的小球藻效应基因，并使用病毒诱导的基因沉默（VIGS）来确定两种受体样激酶（RLK）NbBAK 1和NbSOBIR 1在小球藻效应子识别中的作用。许多Z. triangulate推定的效应子（63个测试的14个）诱导本塞姆氏烟草中的细胞死亡或失绿。对于大多数，表型是光依赖性的，需要效应子分泌到叶质外体空间。此外，效应子诱导的宿主细胞死亡依赖于NbBAK 1和NbSOBIR 1。我们的研究结果表明，广泛认识质外体效应从小麦感染真菌病原体在分类学上遥远的非宿主植物物种推测细胞表面免疫受体。这表明，质外体识别多种非适应性病原体效应可能有助于NHR。另见弗里森对这篇文章的评论，213：7-9。
The fungus Zymoseptoria tritici is a strictly apoplastic, host‐specific pathogen of wheat leaves and causal agent of septoria tritici blotch (STB) disease. All other plants are considered nonhosts, but the mechanism of nonhost resistance (NHR) to Z. tritici has not been addressed previously. We sought to develop Nicotiana benthamiana as a system to study NHR against Z. tritici. Fluorescence microscopy and quantitative reverse transcription polymerase chain reactions were used to establish the interaction between Z. tritici and N. benthamiana. Agrobacterium‐mediated transient expression was used to screen putative Z. tritici effector genes for recognition in N. benthamiana, and virus‐induced gene silencing (VIGS) was employed to determine the role of two receptor‐like kinases (RLKs), NbBAK1 and NbSOBIR1, in Z. tritici effector recognition. Numerous Z. tritici putative effectors (14 of 63 tested) induced cell death or chlorosis in N. benthamiana. For most, phenotypes were light‐dependent and required effector secretion to the leaf apoplastic space. Moreover, effector‐induced host cell death was dependent on NbBAK1 and NbSOBIR1. Our results indicate widespread recognition of apoplastic effectors from a wheat‐infecting fungal pathogen in a taxonomically distant nonhost plant species presumably by cell surface immune receptors. This suggests that apoplastic recognition of multiple nonadapted pathogen effectors may contribute to NHR. See also the Commentary on this article by Friesen, 213: 7–9.
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