Differential regulation of interleukin-6 receptor and gp130 gene expression in rat hepatocytes.
Differential regulation of interleukin-6 receptor and gp130 gene expression in rat hepatocytes.
复制标题
大鼠肝细胞中白细胞介素 6 受体和 gp130 基因表达的差异调节。
DOI：
10.1091/mbc.3.1.103
复制
发表时间：
1992
影响因子：
3.3
通讯作者：
Fuller,GM
中科院分区：
文献类型：
--
作者：
Nesbitt,JE;Fuller,GM
白细胞介素-6（IL-6）在急性炎症反应的早期阶段向肝细胞传递重要信号，导致几种主要防御蛋白表达的改变。通过改变IL-6受体（IL-6-R）或信号转导蛋白gp 130的数量，可以对该信号进行额外的调节。我们采用核糖核酸酶保护试验来测量IL-6、白细胞介素-1、地塞米松及其组合在原代大鼠肝细胞中的IL-6-R和gp 130 mRNA的表达。地塞米松增加受体mRNA水平2.7倍以上的控制，但没有检测到的影响，gp 130。这种处理使IL-6-R的表面表达从每个细胞600个受体增加到大于6000个受体，而Kd（2.5-4.6 × 10（-10）M）没有变化。与类固醇信号的刺激作用相反，炎性细胞因子单独和一起下调IL-6-R的mRNA和细胞表面表达。这些发现首次证明了炎症介质和IL-6-R之间存在敏感的控制系统。
Interleukin-6 (IL-6) relays an important signal to hepatocytes during the early stages of an acute inflammatory response, causing an alteration in the expression of several major defense proteins. Additional regulation of this signal could occur either by altering the number of IL-6 receptors (IL-6-R) or of the signal transducing protein, gp130. We employed ribonuclease protection assays to measure the expression of IL-6-R and gp130 mRNA in primary rat hepatocytes in response to IL-6, interleukin-1, dexamethasone, and combinations thereof. Dexamethasone increases receptor mRNA levels 2.7-fold above controls but has no detectable effect on that of gp130. Such treatment increased surface expression of IL-6-R from 600 receptors per cell to greater than 6000, without a change in Kd (2.5-4.6 x 10(-10) M). In contrast to the stimulatory effect of the steroid signal, the inflammatory cytokines, individually and together, down-modulated both the mRNA and the cell surface expression of IL-6-R. These findings demonstrate for the first time that a sensitive control system exists between inflammatory mediators and IL-6-R.