Oligonucleotide probes to the 5' end of the framework 3 (FR3) gene segment detect polymorphisms of VH gene sequences encoding biologically important amino acid residues.
Oligonucleotide probes to the 5' end of the framework 3 (FR3) gene segment detect polymorphisms of VH gene sequences encoding biologically important amino acid residues.
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框架 3 (FR3) 基因片段 5 端的寡核苷酸探针可检测编码生物学重要氨基酸残基的 VH 基因序列的多态性。
DOI：
10.1111/j.1365-3083.1993.tb01661.x
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发表时间：
1993
影响因子：
3.7
通讯作者：
Guillaume,T
中科院分区：
文献类型：
--
作者：
Rubinstein,DB;Symann,M;Guillaume,T
在这项研究中，我们证明了使用寡核苷酸探针的最VH家族特异性的框架，ER3（ER3）基因片段，在生殖系DNA的分析。与限制性内切酶消化的基因组DNA与大的克隆的人VH家族探针的杂交相比，与来自ER 3基因片段的核苷酸199至258的寡核苷酸的杂交既产生较不复杂的模式，又突出个体之间的多态性变异。由FR 3基因片段编码的氨基酸残基在影响物理上邻近的互补决定区（CDR）的构象、抗原结合和亲和力中发挥的潜在重要作用表明FR 3序列保守性具有生物学意义。结合来自CDR和框架限制性片段多态性的信息的基因组研究可以证明在检测特定基因和鉴定感兴趣的单倍型中是有用的。
In this study, we demonstrate the use of oligonucleotide probes to the most VH family‐speeific framework, the Eramework 3 (ER3) gene segment, in the analysis of germline DNA. Compared with the hybridization of restriction enzyme‐digested genomic DNA to large, cloned human VH family probes, hybridization with oligonueleotides derived from nucleotides 199 to 258 of the ER3 gene segments both yields less complex patterns and highlights polymorphic variations between individuals. The potentially important role played by amino acid residues encoded by the FR3 gene segment in influencing conformation, antigen binding and affinity of the physically proximal complementarity determining regions (CDRs) suggests FR3 sequence conservation to be biologically significant. Genomic studies combining information from both CDR and framework restriction fragment polymorphisms can prove useful in detecting specific genes and in identifying haplotypes of interest.