Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR
Effective production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR
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DOI：
10.1016/j.jbiotec.2004.08.016
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发表时间：
2005-02-09
影响因子：
4.1
通讯作者：
Kumagai, H
中科院分区：
文献类型：
--
作者：
Koyanagi, T;Katayama, T;Kumagai, H
利用Erwinia除草剂细胞酶促生产3,4-二羟基苯基-l -丙氨酸（L-DOPA）涉及酪氨酸苯酚裂解酶的作用（Tpl, EC 4.1.99.2）。由于Tpl仅在l -酪氨酸诱导的条件下合成，因此在制备细胞（酶源）时不可避免地在培养基中添加l -酪氨酸，但严重阻碍了最终产物L-DOPA的纯制备。我们利用携带突变型转录调节因子TyrR的重组E型除草剂细胞规避了这一问题，该基因能够在缺乏l -酪氨酸的情况下激活tpl启动子。(C) 2004 Elsevier B.V.版权所有
The enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) using Erwinia herbicola cells involves the action of tyrosine phenol-lyase (Tpl, EC 4.1.99.2). Since Tpl is only synthesized under L-tyrosine-induced conditions, the addition of L-tyrosine to the medium is unavoidable when preparing cells (the enzyme source), but severely impedes the pure preparation of the final product L-DOPA. We circumvented this problem by using recombinant E herbicola cells carrying a mutant transcriptional regulator TyrR, which is capable of activating the tpl promoter in the absence of L-tyrosine. (C) 2004 Elsevier B.V. All rights reserved.