Consumption of selenium-enriched broccoli increases cytokine production in human peripheral blood mononuclear cells stimulated ex vivo, a preliminary human intervention study
Consumption of selenium-enriched broccoli increases cytokine production in human peripheral blood mononuclear cells stimulated ex vivo, a preliminary human intervention study
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DOI：
10.1002/mnfr.201400438
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发表时间：
2014-12-01
影响因子：
5.2
通讯作者：
McKenzie, Marian J.
中科院分区：
文献类型：
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作者：
Bentley-Hewitt, Kerry L.;Chen, Ronan K. -Y.;McKenzie, Marian J.
范围：硒（Se）是人体健康所必需的微量营养素，包括免疫功能。先前的研究表明，硒补充可能导致T辅助（Th）1-型免疫反应向th2型免疫反应转变。我们的目的是测试富硒西兰花的潜在健康促进作用。方法和结果：在一项人体试验中，18名参与者连续3天每天食用对照西兰花。在3天的洗脱期后，参与者连续3天食用每份含硒200毫克的富硒西兰花。在每个摄食期开始和结束时采集血浆和外周血单个核细胞（PBMC）样品，分析PBMC体内总硒含量，并测定抗原刺激下PBMC细胞因子的产量。血浆硒含量在整个对照期与富硒期基线（1.22 μ mol/L）保持一致，富硒3 d后显著升高。饲喂富硒西兰花3天后，PBMC的白细胞介素（IL-2、IL-4、IL-5、IL-13和IL-22）产量显著高于基线水平。结论：本研究表明，食用富含硒的西兰花可能会增加对一系列免疫挑战的免疫反应。
Scope: Selenium (Se) is a micronutrient essential for human health, including immune function. Previous research indicates that Se supplementation may cause a shift from T helper (Th)1- to Th2-type immune responses. We aim to test the potential health promoting effects of Se-enriched broccoli.Methods and results: In a human trial, 18 participants consumed control broccoli daily for 3 days. After a 3-day wash-out period, the participants were provided with Se-enriched broccoli containing 200 mu g of Se per serving for 3 days. Plasma and peripheral blood mononuclear cell (PBMC) samples were collected at the start and end of each broccoli feeding period for analysis of total Se and measurement of cytokine production from PBMC stimulated with antigens ex vivo. Plasma Se content remained consistent throughout the control broccoli feeding period and the baseline of the Se-enriched broccoli period (1.22 mu mol/L) and then significantly increased following 3 days of Se-enriched broccoli feeding. Interleukin (IL-2, IL-4, IL-5, IL-13, and IL-22) production from PBMC significantly increased after 3 days of Se-enriched broccoli feeding compared with baseline.Conclusion: This study indicates that consumption of Se-enriched broccoli may increase immune responses toward a range of immune challenges.