Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
复制标题
果蝇病毒感染的建立和宿主-病毒相互作用的分析。
DOI：
10.3791/58845
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lei Pan
中科院分区：
文献类型：
--
作者：
Shuo Yang;Yaya Zhao;Junjing Yu;Zhiqin Fan;Si-tang Gong;Hong Tang;Lei Pan
病毒传播是流行病的主要原因。因此，了解病毒与宿主之间的相互作用对于扩展我们对病毒感染的预防和治疗的知识是非常重要的。果蝇（Drosophila melanogaster）由于其强大的遗传工具和高度保守的先天免疫信号通路，已被证明是筛选抗病毒因子和研究病毒-宿主相互作用的最有效和多产的模式生物之一。本文描述的程序证明了纳米注射方法在成年果蝇中建立病毒感染并诱导全身抗病毒反应。该方法中病毒注射剂量的精确控制使得实验重现性高。本研究中描述的方案包括苍蝇和病毒的制备、注射方法、存活率分析、病毒载量测量和抗病毒途径评估。本文还讨论了蝇类本底对病毒感染的影响。这种感染方法易于执行和定量可重复的，它可以应用于筛选参与病毒-宿主相互作用的宿主/病毒因子，并剖析先天免疫信号传导和其他生物学途径之间的串扰响应病毒感染。
Virus spreading is a major cause of epidemic diseases. Thus, understanding the interaction between the virus and the host is very important to extend our knowledge of prevention and treatment of viral infection. The fruit fly Drosophila melanogaster has proven to be one of the most efficient and productive model organisms to screen for antiviral factors and investigate virus-host interaction, due to powerful genetic tools and highly conserved innate immune signaling pathways. The procedure described here demonstrates a nano-injection method to establish viral infection and induce systemic antiviral responses in adult flies. The precise control of the viral injection dose in this method enables high experimental reproducibility. Protocols described in this study include the preparation of flies and the virus, the injection method, survival rate analysis, the virus load measurement, and an antiviral pathway assessment. The influence effects of viral infection by the flies' background were mentioned here. This infection method is easy to perform and quantitatively repeatable; it can be applied to screen for host/viral factors involved in virus-host interaction and to dissect the crosstalk between innate immune signaling and other biological pathways in response to viral infection.