Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins
Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins
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DOI：
10.1074/mcp.m300079-mcp200
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发表时间：
2003-12-01
影响因子：
7
通讯作者：
Jensen, ON
中科院分区：
文献类型：
--
作者：
Elortza, F;Nühse, TS;Jensen, ON
糖基磷脂酰肌醇锚定蛋白（GPI-AP）是一个功能和结构多样的后修饰膜蛋白家族，主要存在于多种真核细胞的质膜外叶中。虽然GPI-AP的一般作用仍不清楚，但它们已引起注意，因为它们在细胞粘附、分化和宿主-病原体相互作用中充当酶和受体。GPI-AP可能代表人类潜在的诊断和治疗靶点，并且由于其在根发育中的关键作用而在植物生物技术中引起兴趣。在这里，我们提出了一个一般的质谱为基础的蛋白质组学的“剃和征服”的战略，专门针对GPI-AP。使用生物化学方法，质谱和计算序列分析的组合，我们确定了6 GPI-AP在智人脂筏富集馏分和44 GPI-AP在拟南芥膜制备，代表迄今为止最大的实验数据集GPI锚定蛋白。
Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are a functionally and structurally diverse family of post-translationally modified membrane proteins found mostly in the outer leaflet of the plasma membrane in a variety of eukaryotic cells. Although the general role of GPI-APs remains unclear, they have attracted attention because they act as enzymes and receptors in cell adhesion, differentiation, and host-pathogen interactions. GPI-APs may represent potential diagnostic and therapeutic targets in humans and are interesting in plant biotechnology because of their key role in root development. We here present a general mass spectrometry-based proteomic "shave-and-conquer" strategy that specifically targets GPI-APs. Using a combination of biochemical methods, mass spectrometry, and computational sequence analysis we identified six GPI-APs in a Homo sapiens lipid raft-enriched fraction and 44 GPI-APs in an Arabidopsis thaliana membrane preparation, representing the largest experimental dataset of GPI-anchored proteins to date.