Direct observation of ligand transfer and bond formation in cytochrome c oxidase by using mid-infrared chirped-pulse upconversion
Direct observation of ligand transfer and bond formation in cytochrome c oxidase by using mid-infrared chirped-pulse upconversion
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DOI：
10.1073/pnas.0703279104
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发表时间：
2007-10-02
影响因子：
11.1
通讯作者：
Alexandrou, Antigoni
中科院分区：
文献类型：
--
作者：
Treuffet, Johanne;Kubarych, Kevin J.;Alexandrou, Antigoni
我们已经在可见光泵浦中红外探针实验中实现了最近展示的将中红外飞秒脉冲啁啾脉冲上转换为可见光的技术，以在宽光谱范围内进行高分辨率、高灵敏度测量。我们成功地解决了哺乳动物细胞色素c氧化酶双金属活性位点中从血红素Fe到邻近的Cu-B原子的CO配体转移过程的时间分辨，已知该过程在
We have implemented the recently demonstrated technique of chirped-pulse upconversion of midinfrared femtosecond pulses into the visible in a visible pump-midinfrared probe experiment for high-resolution, high-sensitivity measurements over a broad spectral range. We have succeeded in time-resolving the CO ligand transfer process from the heme Fe to the neighboring Cu-B atom in the bimetallic active site of mammalian cytochrome c oxidase, which was known to proceed in