Regulation of long-range BMP gradients and embryonic polarity by propagation of local calcium-firing activity.
Regulation of long-range BMP gradients and embryonic polarity by propagation of local calcium-firing activity.
复制标题
通过传播局部钙激发活性来调节远程 BMP 梯度和胚胎极性。
DOI：
10.1038/s41467-024-45772-4
复制
发表时间：
2024
影响因子：
16.6
通讯作者：
Stern,ClaudioD
中科院分区：
文献类型：
--
作者：
Lee,HyungChul;Oliveira,NidiaMM;Hastings,Cato;Baillie-Benson,Peter;Moverley,AdamA;Lu,Hui-Chun;Zheng,Yi;Wilby,EliseL;Weil,TimothyT;Page,KarenM;Fu,Jianping;Moris,Naomi;Stern,ClaudioD
包括人类在内的许多脊椎动物都可以从一个胚胎中形成同卵双胞胎，但这种情况很少发生。有人提出，形成连续条纹的胚胎区域发出信号，抑制其他地方的条纹形成，但所涉及的信号，它们是如何传递的，以及它们是如何起作用的还没有阐明。在这里，我们表明，短轨道的钙放电活动传播通过胚外组织通过缝隙连接，并防止异位原条形成鸡胚。通过NF-κB和NFAT对钙活性和原条形成抑制剂（骨形态发生蛋白，BMP）的交叉调节建立了跨越胚胎的长程BMP梯度。这一机制解释了不同大小的胚胎如何保持决定胚胎极性的位置信息。我们在两个不同的人类胚胎模型和果蝇中提供了类似机制的证据，表明了一个古老的进化起源。
Many amniote vertebrate species including humans can form identical twins from a single embryo, but this only occurs rarely. It has been suggested that the primitive-streak-forming embryonic region emits signals that inhibit streak formation elsewhere but the signals involved, how they are transmitted and how they act has not been elucidated. Here we show that short tracks of calcium firing activity propagate through extraembryonic tissue via gap junctions and prevent ectopic primitive streak formation in chick embryos. Cross-regulation of calcium activity and an inhibitor of primitive streak formation (Bone Morphogenetic Protein, BMP) via NF-κB and NFAT establishes a long-range BMP gradient spanning the embryo. This mechanism explains how embryos of widely different sizes can maintain positional information that determines embryo polarity. We provide evidence for similar mechanisms in two different human embryo models and inDrosophila, suggesting an ancient evolutionary origin.