Antifungal activity and mode of action of silver nano-particles on Candida albicans
Antifungal activity and mode of action of silver nano-particles on Candida albicans
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DOI：
10.1007/s10534-008-9159-2
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发表时间：
2009-04-01
期刊：
影响因子：
3.5
通讯作者：
Lee, Dong Gun
中科院分区：
文献类型：
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作者：
Kim, Keuk-Jun;Sung, Woo Sang;Lee, Dong Gun
在这项研究中，研究了银纳米颗粒（nano-Ag）的抗真菌作用及其作用方式。纳米银对真菌具有抗真菌作用，经测试对人类红细胞具有低溶血作用。为了阐明纳米银的抗真菌作用模式，对白色念珠菌进行了流式细胞术分析、葡萄糖释放测试、透射电子显微镜 (TEM) 以及使用 1,6-二苯基-1,3,5-己三烯 (DPH) 作为质膜探针的膜动力学变化。结果表明，纳米银可能通过破坏细胞膜的结构并由于膜完整性的破坏而抑制正常的出芽过程而发挥抗真菌活性。目前的研究表明纳米银具有相当大的抗真菌活性，值得进一步研究其临床应用。
In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.