Effects of Prepubertal or Adult Site-Specific Knockdown of Estrogen Receptor β in the Medial Preoptic Area and Medial Amygdala on Social Behaviors in Male Mice.
Effects of Prepubertal or Adult Site-Specific Knockdown of Estrogen Receptor β in the Medial Preoptic Area and Medial Amygdala on Social Behaviors in Male Mice.
复制标题
雌激素受体β的前后雌激素受体β和内侧杏仁核对雄性小鼠社交行为的影响。
DOI：
10.1523/eneuro.0155-15.2016
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发表时间：
2016-03
期刊：
影响因子：
3.4
通讯作者：
Ogawa S
中科院分区：
文献类型：
--
作者：
Nakata M;Sano K;Musatov S;Yamaguchi N;Sakamoto T;Ogawa S
睾酮在大脑中转化为雌二醇后，作用于雌激素受体（ERα和ERβ）并控制男性型社会行为的表达。雄性小鼠的研究表明，雌激素受体α（ERα）表达于内侧视前区（MPOA）和内侧杏仁核（MeA），在成年期和青春期分别参与睾酮对性行为和攻击行为的调节。然而，ERβ在这些大脑区域中发挥的作用仍然不清楚。本研究采用腺相关病毒介导的RNA干扰技术，在ICR/Jcl小鼠中研究了MPOA和MeA中ERβ（βERKD）的位点特异性敲低对雄性社会行为的影响。MPOA中青春期前的βERKD显示，在整个青春期和成年期持续抑制ERβ基因表达可减少攻击性行为，但成年后的性行为测试结果不显示这一点。由于MPOA中的βERKD仅在成年期不影响性行为或攻击行为，因此认为MPOA中的青春期ERβ可能对成年期攻击行为的充分表达起重要作用。另一方面，尽管青春期前和成年期MeA中的βERKD对性行为和攻击行为都没有任何影响，但成年期βERKD破坏了接受性雌性对非接受性雌性的性偏好。总之，这些结果表明MPOA和MeA中的ERβ以与ERα显著不同的方式参与男性性行为和攻击行为的调节。
Testosterone, after being converted to estradiol in the brain, acts on estrogen receptors (ERα and ERβ) and controls the expression of male-type social behavior. Previous studies in male mice have revealed that ERα expressed in the medial preoptic area (MPOA) and medial amygdala (MeA) are differently involved in the regulation of sexual and aggressive behaviors by testosterone action at the time of testing in adult and/or on brain masculinization process during pubertal period. However, a role played by ERβ in these brain regions still remains unclear. Here we examined the effects of site-specific knockdown of ERβ (βERKD) in the MPOA and MeA on male social behaviors with the use of adeno-associated viral mediated RNA interference methods in ICR/Jcl mice. Prepubertal βERKD in the MPOA revealed that continuous suppression of ERβ gene expression throughout the pubertal period and adulthood decreased aggressive but not sexual behavior tested as adults. Because βERKD in the MPOA only in adulthood did not affect either sexual or aggressive behaviors, it was concluded that pubertal ERβ in the MPOA might have an essential role for the full expression of aggressive behavior in adulthood. On the other hand, although neither prepubertal nor adult βERKD in the MeA had any effects on sexual and aggressive behavior, βERKD in adulthood disrupted sexual preference of receptive females over nonreceptive females. Collectively, these results suggest that ERβ in the MPOA and MeA are involved in the regulation of male sexual and aggressive behavior in a manner substantially different from that of ERα.