Singlet oxygen mutagenicity induced in the lac operon.
Singlet oxygen mutagenicity induced in the lac operon.
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紫胶操纵子中诱导的单线态氧致突变性。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
A. Van de Vorst
中科院分区：
文献类型：
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作者：
D. Decuyper;J. Piette;M. Jassogne;A. Van de Vorst
我们通过分析m13mp19杂交噬菌体lac插入片段的o2诱导突变，研究了单线态氧（1O2）诱变单链DNA噬菌体的特异性。107个lac突变体以单碱基替换为主，其中40-50个碱基缺失突变占93%和7%。大部分的替换是G----T和C----A的转换，分别占突变的27%和54%。以m13mp19 DNA的复制形式（RFDNA）作为底物进行1O2反应，产生两种类型的噬菌体DNA。由于鸟嘌呤残基是氧化的目标，两种类型的转化似乎是由一种损伤提供的：被1O2氧化的鸟嘌呤像胸腺嘧啶一样被大肠杆菌DNA聚合酶i读取。
We have studied the specificity of singlet oxygen (1O2) mutagenesis in single-stranded DNA phage by analysing 1O2-induced mutations in the lac insert of the M13 mp 19 hybrid phage. 107 lac mutants were analysed showing mainly single-base substitutions with a total of 93% and 7% of 40-50 base deletion mutations. Most of the substitutions are G----T and C----A transversions with respectively 27 and 54% of the mutations. The replicative form of the M13 mp 19 DNA (RFDNA) was used as substrate for the 1O2 reactions, there are then two types of progeny phages DNA's. As guanine residues are the targets of the oxidation, it appears that both types of transversions are provided by one type of lesion: the guanine oxidised by 1O2 is read like a thymine by E. coli DNA polymerase-I.