Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification.
Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification.
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DOI：
10.1155/2011/780836
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发表时间：
2011
影响因子：
--
通讯作者：
Kanai Y
中科院分区：
文献类型：
--
作者：
Gotoh M;Arai E;Wakai-Ushijima S;Hiraoka N;Kosuge T;Hosoda F;Shibata T;Kondo T;Yokoi S;Imoto I;Inazawa J;Kanai Y
为建立胰腺导管腺癌的诊断标准，采用基于细菌人工染色体(BAC)阵列的甲基化CpG岛扩增技术对139例胰腺癌组织标本进行了检测。12个BAC克隆的DNA甲基化状态能够区分学习队列中的癌组织(T)和非癌组织(T)，特异性为100%。使用结合12个BAC克隆的标准，T样本被诊断为癌症，在学习和验证队列中都具有100%的敏感性和特异性。在学习队列中，11个BAC克隆上的DNA甲基化状态能够以100%的特异性区分早期复发和未复发的患者，与验证队列中的无复发和总生存率相关，并通过多因素分析成为独立的预后因素。全基因组DNA甲基化分析可能为PC患者提供最佳的诊断标记物和预后指标。
To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100%, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100% sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100% specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.
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