Increasing fetal adrenal formation of cortisol from pregnenolone during baboon (Papio papio) gestation.
Increasing fetal adrenal formation of cortisol from pregnenolone during baboon (Papio papio) gestation.
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在狒狒 (Papio papio) 妊娠期间，通过孕烯醇酮增加胎儿肾上腺皮质醇的形成。
DOI：
10.1095/biolreprod17.5.701
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发表时间：
1977
影响因子：
3.6
通讯作者：
J. D. Townsley
中科院分区：
文献类型：
--
作者：
G. Pepe;J. A. Titus;J. D. Townsley
为了确定狒狒新生儿中导致肾上腺皮质自给自足的成熟变化，在妊娠期间的不同时间检查了胎儿肾上腺生长和体外由双烯醇酮（P5）、孕酮（P4）和11-脱氧皮质醇（S）产生的皮质醇（F）。提前分娩动物的肾上腺重量（妊娠100 - 179天，足月= 184）剖宫产（N = 17）或足月时自发（N = 13）（181 ± 4天，X ± SE），在妊娠后半期增加了两倍（r = 0.82; P <0.001; Y = 403e0.017X），主要是由于皮质胎儿区组织的生长。在提取和纯化[3H1F]后，测定50 mg来自与NADPH孵育的选定腺体的匀浆组织的[3H1P5]、[3H1P4]或[13HIS]（1512 Ci，101 μ g）的F产生。结果来自于84至54天（N = 4）或足月前24至17天（N = 3）通过剖宫产分娩的动物以及来自于出生当天（N = 3）或出生12天（N = 2）的新生儿。从S生产F（68.2% ± 13.1%，X ± SE）和P4（42.4% ± 2.9%）在研究期间保持不变或下降，而P5（6.9% ± 1.9%）增加从孕中期的3 ± 1 pmole/mg到足月前的14 ± 5 pmole/mg，自然分娩后第1天达到51 ± 13 pmole/mg，差异有显著性（P <0.001）。经肾上腺重量校正后，S（88 - 118 nmole/2肾上腺）和P4（46 - 77 nmole/2肾上腺）的F产生量保持不变，而P5的F产生量从妊娠中期的0.3 ± 0.1 nmole/2肾上腺增加到自然分娩后第1天的17 ± 5 nmole/2肾上腺（P <0.001）。这种能力在出生后的前12天一直保持着。因此，胎儿肾上腺313-羟类固醇脱氢酶异构酶活性增加，而11 β-，17 n-和21-羟化酶活性相对较大，但在妊娠期间不变。它的结论是，增加3J3-羟基类固醇脱氢酶异构酶结合肾上腺生长允许增加利用P5的F合成对长期。这些变化可能是重要的，在确保肾上腺皮质自给自足时，分娩后，胎盘P4不再可用于F生产。
To determine the maturational changes leading to adrenocortical self-sufficiency in baboon neonates, fetal adrenal growth and cortisol (F) production, in vitro, from pregnenolone (P5), progesterone (P4) and 11-deoxycortisol (S) were examined at various times during gestation. Adrenal weight of animals delivered prematurely (100-179 days gestation, term = 184) by cesarean section (N = 17) or spontaneously (N = 13) at term (181 ± 4 days, X ± SE), tripled during the second half of gestation (r = 0.82; P<0.0O1; Y = 403e0.0I7X) and resulted primarily from growth of cortical fetal zone tissue. F production from [3H1P5, [3H1P4 or I3HIS(1512Ci, lOlhg) by 50 mg homogenized tissue from selected glands incubated with NADPH was determined following extraction, and purification of [3 HI F. Resultswere combined from animals deliveredby cesarean section 84 to 54 days (N = 4), or 24 to 17 days prior to term (N = 3)and from neonates iday old (N = 3) or Sand 12 days old (N = 2). F production from S (68.2 percent ± 13.1 percent, X ± SE) and P4 (42.4 percent ± 2.9 percent) either remained constant or decreased during the study period, while that from P5 (6.9 percent ± 1.9 percent) increased (P<0.001) from 3 ± I pmole/mg at midgestation to 14 ± 5 pmole/mg shorly before term, reaching 51 ± 13 pmole/mg 1 day after spontaneous delivery. When corrected for adrenal weight, F production from S (88-118 nmole/2 adrenals) and P4 (46-77 nmole/2 adrenals) remained constant whereas that from P5 increased (P<0.001) from 0.3 ± 0.1 nmole/2 adrenals at midgestation to 17 ± 5 nmole/2 adrenalsI day after spontaneous delivery. This capacity was maintained in the first 12 days of life. Thus fetal adrenal 313-hydroxysteroid dehydrogenase-isomerase activity is increased, while 11(3-,17n- and 21-hydroxylase activities are relatively greater but unchanged during gestation. It is concluded that increased 3j3-hydroxysteroid dehydrogenase-isomerase combined with adrenal growth permits increased utilization of P5 for F synthesis towards term. These changes may be important in ensuring adrenocortical self-sufficiency when, after delivery, placental P4 is no longer available for F production.