Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells
Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells
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DOI：
10.1007/s11010-011-0828-z
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Sedo, Aleksi
中科院分区：
文献类型：
--
作者：
Balaziova, Eva;Busek, Petr;Sedo, Aleksi
二肽基肽酶-IV（DPP-IV）和成纤维细胞活化蛋白-α（FAP）由于其独特的酶活性以及通过非水解分子相互作用而被推测参与多种生物过程的调节。目前，DPP-IV和FAP在包括胶质母细胞瘤在内的各种类型的肿瘤的发展和进展中的作用被深入研究，并且它们的功能串扰被假设。在这篇文章中，我们描述了相关的表达DPP-IV和FAP mRNA的原代细胞培养来自人胶质母细胞瘤和相关的表达动力学的两种分子在星形细胞瘤细胞系取决于培养条件。虽然DPP-IV和FAP共调节的分子机制仍不清楚，但转基因DPP-IV和内源FAP的非偶联表达表明它发生在转录水平而不是转录后水平。了解DPP-IV和FAP在表达和功能上的协调关系有助于阐明这两种分子在转化星形胶质细胞中的生物学作用机制。
Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein-alpha (FAP) are speculated to participate in the regulation of multiple biological processes, because of their unique enzymatic activity, as well as by non-hydrolytic molecular interactions. At present, the role of DPP-IV and FAP in the development and progression of various types of tumors, including glioblastoma, is intensively studied, and their functional crosstalk is hypothesized. In this article, we describe the correlative expression of DPP-IV and FAP mRNA in primary cell cultures derived from human glioblastoma and associated expression dynamics of both molecules in astrocytoma cell lines depending on culture conditions. Although the molecular mechanisms of DPP-IV and FAP co-regulations remain unclear, uncoupled expression of transgenic DPP-IV and the endogenous FAP suggests that it occurs rather at the transcriptional than at the posttranscriptional level. Understanding of the expressional and functional coordinations of DPP-IV and FAP may help clarify the mechanisms of biological roles of both molecules in transformed astrocytic cells.