Identification of a subset of normal B cells with a Burkitt's lymphoma (BL)-like phenotype.
Identification of a subset of normal B cells with a Burkitt's lymphoma (BL)-like phenotype.
复制标题
鉴定具有伯基特淋巴瘤 (BL) 样表型的正常 B 细胞亚群。
作者：
C. Gregory;T. Tursz;C. Edwards;C. Tétaud;M. Talbot;B. Caillou;A. Rickinson;M. Lipinski
来自伯基特淋巴瘤（BL）病例的新鲜活检细胞显示同质的细胞表面表型。发现这些细胞与全B细胞标记物B1反应，并始终共表达BL相关糖脂抗原BLA和常见急性淋巴细胞白血病抗原CALLA，但缺乏EB病毒转化的正常B细胞上特征性表达的B细胞“活化”抗原。从一系列新鲜正常扁桃体中分离的细胞的显微镜和细胞分选仪分析已经鉴定出携带相同细胞表面标记的正常B细胞亚群。扁桃体悬液双色免疫荧光（IF）和连续切片免疫过氧化物酶（IP）染色证实BLA和CALLA可在单个B细胞上共表达。这些BLA+、CALLA+、“活化”抗原-细胞进一步表征为B1+、sIgM+、sIgD-、C3 d/EB病毒受体+，并且在体外对病毒诱导的转化敏感。Percoll法分离的扁桃体细胞群的IF研究和IP染色的扁桃体半薄切片的直接检查表明BLA+、CALLA+细胞定位于生发中心。它们的形态特征与BL细胞相匹配，并且它们在生发中心内的位置与已知的生发中心扁桃体B细胞的表型和BL作为中心母细胞增殖的描述一致。我们认为扁桃体生发中心B细胞是BL肿瘤细胞的正常对应物。
Fresh biopsy cells from cases of Burkitt's lymphoma (BL) display a homogeneous cell surface phenotype. The cells were found to be reactive with the pan B cell marker B1, and consistently co-expressed the BL-associated glycolipid antigen, BLA, and the common acute lymphoblastic leukemia antigen, CALLA, but lacked the B cell "activation" antigens characteristically expressed on EB virus-transformed normal B cells. Microscopic and cell sorter analysis of cells isolated from a series of fresh normal tonsils have identified a subpopulation of normal B cells carrying the same cell surface markers. That BLA and CALLA could be co-expressed on individual B cells was demonstrated by two-color immunofluorescence (IF) of tonsils in suspension, and immunoperoxidase (IP) staining of serial tonsil sections. These BLA+, CALLA+, "activation" antigen- cells were further characterized as B1+, sIgM+, sIgD-, C3d/EB virus receptor+ and were susceptible to virus-induced transformation in vitro. IF studies on Percoll-fractionated tonsillar cell populations and direct examination of IP-stained tonsil semi-thin sections indicated that the BLA+, CALLA+ cells were localized in germinal centers. Their morphological characteristics matched those of BL cells, and their location within germinal centers was consistent both with the known phenotype of germinal center tonsillar B cells and with the description of BL as a proliferation of centroblasts. We suggest that this population of tonsillar germinal center B cells provides the normal counterpart of BL tumor cells.