Highly discriminatory typing method for Listeria monocytogenes using polymorphic tandem repeat regions
Highly discriminatory typing method for Listeria monocytogenes using polymorphic tandem repeat regions
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DOI：
10.1016/j.mimet.2012.05.023
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发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Kimura, Bon
中科院分区：
文献类型：
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作者：
Miya, Satoko;Takahashi, Hajime;Kimura, Bon
串联重复序列（Tandem repeats，TR）是DNA中重复的核苷酸序列，在重复序列的数量和序列上都具有多态性。在这项研究中，我们开发了一种新的分型方法，多位点TR序列分析（MLTSA），为食源性病原体单核细胞增生李斯特菌在三个串联重复序列多态性。聚类分析表明，L.分别对谱系I和谱系II的单核细胞增生性李斯特菌菌株进行聚类，并在谱系I聚类内形成三个组，每个组包括三个主要单核细胞增生性李斯特菌流行性克隆（ECI、ECIa和ECII）中的一个。这些结果与先前建立的基于毒力基因的MLST方法一致。相比之下，我们的方法将一些流行病学相关的分离株分组在一起，而基于毒力基因的MIST没有。此外，我们的方法，使用三个串联重复区域，表现出更高的判别力比MLST方法，它使用六个毒力基因区域。这种利用TR区序列多态性的MLTSA方法可能是一种有用的工具，用于L。单核细胞增多症(C)2012爱思唯尔有限公司版权所有。
Tandem repeats (TR), which are repetitive nucleotide sequences in DNA, are polymorphic both in repeat number and sequence. In this study, we developed a new typing method, multilocus TR sequence analysis (MLTSA), for the foodborne pathogen Listeria monocytogenes using sequence polymorphisms in three tandem repeat regions. The obtained dendrogram clustered L. monocytogenes strains of lineage I and lineage II separately, and formed three groups within the lineage I cluster, each of which included one of the three major L monocytogenes epidemic clones (ECI, ECIa, and ECII). These results were consistent with a previously established virulence-gene-based MLST method. In comparison, our method grouped some epidemiologically related isolates together, which virulence-gene-based MIST did not. Moreover, our method, using three tandem repeat regions, showed a higher discriminatory power than the MLST method, which uses six virulence gene regions. This MLTSA approach using sequence polymorphisms in TR regions could be a useful tool in the epidemiological study of L. monocytogenes. (C) 2012 Elsevier B.V. All rights reserved.