Increase in bone metabolic markers and circulating osteoblast-lineage cells after orthognathic surgery
Increase in bone metabolic markers and circulating osteoblast-lineage cells after orthognathic surgery
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DOI：
10.1038/s41598-019-56484-x
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发表时间：
2019-12-27
影响因子：
4.6
通讯作者：
Takahashi, Tetsu
中科院分区：
文献类型：
--
作者：
Abe, Yoko;Chiba, Mirei;Takahashi, Tetsu
手术或骨折后矿化率和骨形成增加是局部加速现象（RAP），其全身影响是全身加速现象（SAP）。外周血中循环成骨细胞谱系细胞（包括骨钙素阳性（OCN+）细胞）的比例在青春期生长期间和骨折患者中明显较高。本研究旨在通过纵向前瞻性观察，阐明正颌手术后骨代谢活性的动态变化。采集行正颌手术患者的外周静脉血，测定血清骨代谢指标和OCN+细胞比例。颌骨手术通过靶向骨愈合过程中的步骤和相关蛋白质，如手术应激/炎症（C反应蛋白）、骨吸收（I型胶原C端肽）和骨形成（碱性磷酸酶和骨特异性碱性磷酸酶），诱导骨代谢活性的全身动态变化。在术后早期，OCN+细胞的数量显著增加。共聚焦显微镜显示，OCN蛋白定位于细胞质中的Triton X-100处理的OCN+细胞。此外，在OCN+细胞中检测到OCN、ALP和COL 1A 1基因表达，表明骨髓源性OCN+细胞在骨愈合部位的局部成熟的贡献。
Increased mineralisation rate and bone formation after surgery or fracture is the regional acceleratory phenomenon (RAP), and its systemic impact is the systemic acceleratory phenomenon (SAP). The proportion of circulating osteoblast lineage cells, including osteocalcin-positive (OCN+) cells, in the peripheral blood is markedly higher during pubertal growth and in patients with bone fractures. This study aimed to elucidate the dynamic changes in bone metabolic activity after orthognathic surgery by longitudinal prospective observation. Peripheral venous blood samples were collected from patients who had undergone orthognathic surgery, and serum bone metabolic markers and the proportion of OCN+ cells were measured. Orthognathic surgery induces systemic dynamic changes in bone metabolic activity by targeting steps in the bone healing process and related proteins, such as surgical stress/inflammation (C-reactive protein), bone resorption (type I collagen C-telopeptide), and bone formation (alkaline phosphatase and bone-specific alkaline phosphatase). During the early post-operative period, the population of OCN+ cells significantly increased. Confocal microscopy revealed that OCN proteins were localised in the cytoplasm in Triton X-100-treated OCN+ cells. Furthermore, OCN, ALP, and COL1A1 gene expression was detected in OCN+ cells, suggesting the contribution of the local maturation of bone marrow-derived OCN+ cells at the site of bone healing.