Localized surface plasmon resonance biosensor integrated with microfluidic chip
Localized surface plasmon resonance biosensor integrated with microfluidic chip
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DOI：
10.1007/s10544-009-9306-8
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Borghs, Gustaaf
中科院分区：
文献类型：
--
作者：
Huang, Chengjun;Bonroy, Kristien;Borghs, Gustaaf
基于金纳米颗粒的局域表面等离子共振（LSPR）特性，开发了一种灵敏且低成本的微流控集成生物传感器，可以对生物分子相互作用进行无标记实时监测。引入了一种新颖的象限检测方案，该方案连续测量通过纳米颗粒涂层传感器表面传输的光的变化。使用绿色发光二极管 (LED) 作为光源并结合象限检测方案，确定折射率单位 (RIU) 的分辨率为 10(-4)。该性能可与传统的基于 LSPR 的生物传感器相媲美。使用具有优化的金纳米颗粒薄膜的抗原/抗体（生物素/抗生物素）系统演示了生物传感。通过微流控集成生物传感器定量检测生物素在硫醇基自组装单层 (SAM) 上的固定以及随后抗生物素的亲和结合，并实现了 270 ng/mL 抗生物素的检测限。微流控芯片能够将精确数量的生物样本输送到检测区域，以缩短反应时间和减少试剂消耗，实现高灵敏度和特异的生物传感。将获得的结果与基于表面等离子共振 (SPR) 的 Biacore 系统针对相同结合事件测量的结果进行比较。这项研究证明了基于局域表面等离子体共振的生物传感与微流体技术相结合的可行性，与更大、更昂贵的商业仪器相比，可产生低成本、便携式生物传感器候选物。
A sensitive and low-cost microfluidic integrated biosensor is developed based on the localized surface plasmon resonance (LSPR) properties of gold nanoparticles, which allows label-free monitoring of biomolecular interactions in real-time. A novel quadrant detection scheme is introduced which continuously measures the change of the light transmitted through the nanoparticle-coated sensor surface. Using a green light emitting diode (LED) as a light source in combination with the quadrant detection scheme, a resolution of 10(-4) in refractive index units (RIU) is determined. This performance is comparable to conventional LSPR-based biosensors. The biological sensing is demonstrated using an antigen/antibody (biotin/anti-biotin) system with an optimized gold nanoparticle film. The immobilization of biotin on a thiol-based self-assembled monolayer (SAM) and the subsequent affinity binding of anti-biotin are quantitatively detected by the microfluidic integrated biosensor and a detection limit of 270 ng/mL of anti-biotin was achieved. The microfluidic chip is capable of transporting a precise amount of biological samples to the detection areas to achieve highly sensitive and specific biosensing with decreased reaction time and less reagent consumption. The obtained results are compared with those measured by a surface plasmon resonance (SPR)-based Biacore system for the same binding event. This study demonstrates the feasibility of the integration of LSPR-based biosensing with microfluidic technologies, resulting in a low-cost and portable biosensor candidate compared to the larger and more expensive commercial instruments.