Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa
Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa
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DOI：
10.1016/j.jhazmat.2011.03.095
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发表时间：
2011-06-15
影响因子：
13.6
通讯作者：
Chandrasekaran, Natarajan
中科院分区：
文献类型：
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作者：
Kumari, Mamta;Khan, S. Sudheer;Chandrasekaran, Natarajan
氧化锌纳米颗粒（ZnO NP）在消费品中的使用增加可能会增加其向环境中的释放。植物毒性研究对了解其可能的环境影响具有重要意义。洋葱鳞茎是研究纳米颗粒遗传毒理学的最佳模式生物。本文报道了氧化锌NPs对A. cepa根细胞的细胞遗传学和遗传毒性作用。采用水培法研究了氧化锌NPs对黄颡鱼有丝分裂指数（MI）、微核指数（MN）、染色体畸变指数和脂质过氧化的影响。用四种不同浓度（25、50、75和100 μ g ml(-1)）的ZnO NPs分散体处理茜草根。随着氧化锌NPs浓度的增加，MI随胞浆细胞数量的增加而降低，而MN和染色体畸变指数则升高。与对照组（去离子蒸馏水）相比，ZnO NPs处理的细胞微核细胞的频率更高。氧化锌NPs处理后，各有丝分裂期细胞数量发生变化。通过测量TBARS浓度来检测氧化锌NPs对脂质过氧化的影响，与散装氧化锌相比，在所有浓度下都是明显的。TEM图像显示ZnO NPs样粒子内在化。处理后的cepa的SEM图像表明，内化的纳米颗粒根据细胞内的物理化学环境而聚集。我们的研究结果表明，ZnO NPs可能是一种致裂/遗传毒性和细胞毒性物质。综上所述，cepa细胞遗传学检测可用于氧化锌NPs等新型纳米材料的遗传毒性监测。(C) 2011 Elsevier B.V.版权所有
Increasing use of zinc oxide nanoparticles (ZnO NP) in consumer products may enhance its release into the environment. Phytotoxicity study is important to understand its possible environmental impact. Allium cepa (Onion bulb) is the best model organism to study genetic toxicology of nanoparticles. Here we have reported cytogenetic and genotoxic effects of ZnO NPs on the root cells of A. cepa. The effects of ZnO NPs on the mitotic index (MI), micronuclei index (MN index), chromosomal aberration index, and lipid peroxidation were determined through the hydroponic culturing of A. cepa. A. cepa roots were treated with the dispersions of ZnO NPs at four different concentrations (25, 50, 75, and 100 mu g ml(-1)). With the increasing concentrations of ZnO NPs MI decreased with the increase of pycnotic cells, on the other hand MN and chromosomal aberration index increased. The frequency of micronucleated cells was higher in ZnO NPs treated cells as compared to control (deionized distilled water). The number of cells in each mitotic phase changed upon ZnO NPs treatment. The effect of ZnO NPs on lipid peroxidation as examined by measuring TBARS concentration was evident at all the concentrations compared to bulk ZnO. The TEM image showed internalization of ZnO NPs like particles. SEM image of treated A. cepa demonstrated that the internalized nanoparticles agglomerated depending on the physico-chemical environment inside the cell. Our results demonstrated that ZnO NPs can be a clastogenic/genotoxic and cytotoxic agent. In conclusion, the A. cepa cytogenetic test can be used for the genotoxicity monitoring of novel nanomaterials like ZnO NPs, which is used in many consumer products. (C) 2011 Elsevier B.V. All rights reserved.