ULTRASTRUCTURE OF HUMAN ASTROVIRUS SEROTYPE-2
ULTRASTRUCTURE OF HUMAN ASTROVIRUS SEROTYPE-2
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DOI：
10.1099/0022-1317-76-8-2075
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发表时间：
1995-08-01
影响因子：
3.8
通讯作者：
SANCHEZFAUQUIER, A
中科院分区：
文献类型：
--
作者：
RISCO, C;CARRASCOSA, JL;SANCHEZFAUQUIER, A
通过薄切片和负染色制剂的电子显微镜分析细胞培养物中生长的人星状病毒血清型 2 (H-Ast2) 的超微结构。受感染的 LLCMK2 细胞，如薄片中可见，含有致密或空心颗粒的细胞质聚集体，这些颗粒聚集在准晶体阵列中，并使用兔多克隆抗 Ast2 抗血清进行专门标记。平包埋后薄切片中来自受感染 LLCMK2 细胞上清液的 H-Ast2 颗粒大小与细胞内病毒粒子相似。在阴性染色制剂中，这些病毒颗粒的外径为 41 nm，并表现出清晰的表面尖峰层。五边形和六边形轮廓偶尔可见，可能对应于二十面体结构的投影。仅在 pH 10 下对病毒进行短暂处理后，才在制剂的暗区中看到星状形态和表面三角形空心的颗粒。在 pH 10.5 下孵育病毒会导致病毒颗粒快速分解。具有二十面体几何形状和表面尖刺的颗粒具有完全感染性的发现使得可以提出一种替代传统星状病毒形态学模型的模型。
The ultrastructure of human astrovirus serotype 2 (H-Ast2) grown in cell culture was analysed by electron microscopy of thin sections and negatively stained preparations. Infected LLCMK2 cells, as visualized in thin sections, contained cytoplasmic aggregates of dense or hollow-cored particles that aggregated in quasicrystalline arrays and were specifically labelled using a rabbit polyclonal anti-Ast2 antiserum. H-Ast2 particles from the supernatant of infected LLCMK2 cells in thin sections after flat-embedding were similar in size to intracellular virions. In negatively stained preparations, these virus particles had an external diameter of 41 nm and exhibited a well defined layer of surface spikes. Pentagonal and hexagonal contours were occasionally visible, and probably correspond to the projections of icosahedral structures. Star-like morphologies and particles with surface triangular hollows were seen in dark areas of the preparations only after a short treatment of the viruses at pH 10. Incubation of the viruses at pH 10.5 induced a rapid disassembly of the virus particles. The finding that the particles with icosahedral geometry and surface spikes are fully infective allows an alternative morphological model to the traditional one for astroviruses to be proposed.