The carbohydrate structures of Trypanosoma brucei brucei MITat 1.6 variant surface glycoprotein. A re-investigation of the C-terminal glycan.
The carbohydrate structures of Trypanosoma brucei brucei MITat 1.6 variant surface glycoprotein. A re-investigation of the C-terminal glycan.
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布氏锥虫 MITat 1.6 变体表面糖蛋白的碳水化合物结构。
DOI：
10.1006/bbrc.1993.2412
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发表时间：
1993
影响因子：
3.1
通讯作者：
vanHalbeek,H
中科院分区：
文献类型：
--
作者：
Strang,AM;Allen,AK;Holder,AA;vanHalbeek,H
本文研究了布氏锥虫MIT1.6变异表面糖蛋白（VSG）的寡糖链。通过链霉蛋白酶消化产生糖肽，通过凝胶渗透和离子交换色谱纯化，并通过1H和31 P NMR光谱结合化学组成分析来表征结构。所获得的两种糖肽级分各自被证明在其肽中是均一的，在其碳水化合物结构中是异质的。发现代表VSG的“内部“N-糖基化位点的级分含有与Asn-Ala-Thr连接的结构为Man 7 - 9 GlcNAc 2的高甘露糖型寡糖。另一个糖肽级分含有连接到Asp的VSG的膜锚定C-末端聚糖。其寡糖结构为糖基磷脂酰肌醇（GPI）型：[式]。
We have studied the oligosaccharide chains of the variant surface glycoprotein (VSG) ofTrypanosoma brucei bruceiMITat 1.6. Glycopeptides were generated by Pronase digestion, purified by gel permeation and ion-exchange chromatography, and structurally characterized by1H and31P NMR spectroscopy in combination with chemical composition analyses. The two glycopeptide fractions obtained each proved to be homogeneous in their peptide and heterogeneous in their carbohydrate structures. The fraction representing the "internal"N-glycosylation site of the VSG was found to contain high-mannose type oligosaccharides with structures Man7-9GlcNAc2linked to Asn-Ala-Thr. The other glycopeptide fraction contained the membrane-anchoring C-terminal glycan of the VSG attached to Asp. Its oligosaccharide structures are of the glycosylphosphatidylinositol (GPI) type: [formula].