Overview: engineering transgenic constructs and mice.
Overview: engineering transgenic constructs and mice.
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DOI：
10.1002/0471143030.cb1910s42
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发表时间：
2009-03
影响因子：
--
通讯作者：
Kulkarni, Ashok B
中科院分区：
文献类型：
--
作者：
Haruyama, Naoto;Cho, Andrew;Kulkarni, Ashok B
Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mouse models are now considered essential to correctly understand disease processes in whole animals. This unit provides the basic mouse technologies used to generate conventional transgenic mice, which represents gain-of-function approach. First, an overview of the transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques, and then on typical problems that are encountered when researchers start to generate transgenic mice.
