A direct plaque assay for hog cholera virus.
A direct plaque assay for hog cholera virus.
复制标题
猪霍乱病毒的直接噬菌斑测定。
DOI：
10.1099/0022-1317-40-1-225
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
H. Laude
中科院分区：
文献类型：
--
作者：
H. Laude
用几个猪肾细胞系在琼脂覆盖下获得了猪瘟病毒代表株的直接空斑形成。丙型肝炎病毒感染的细胞在间接光源下看起来像是模糊的斑块，在中性红染色后看起来像白色斑块。直接斑块法检测丙型肝炎病毒快速、简便，其感染力滴度与荧光斑点法相同。
Direct plaque formation with representative strains of hog cholera virus (HCV) has been obtained using several pig kidney cell lines under agar overlay. HCV-infected cells appear as hazy plaques when viewed against an indirect light source, and as white plaques after neutral red staining. HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique.